Anti-PELP1 Polyclonal Antibody for IHC-P, ELISA - Q8IZL8

Core Product Specifications and Parameters

Biological Background: PELP1 Function and Localization

• PELP1 (proline-, glutamic acid- and leucine-rich protein 1), also designated MNAR, P160, or transcription factor HMX3, is a 119.7 kDa scaffold protein that integrates multiple signaling pathways and transcriptional complexes.
• It functions as a coactivator of estrogen receptor-mediated transcription and enables non‑genomic ER signaling through interactions with SRC and PI3K. Related references: PMID:14963108
• PELP1 is an essential component of the nucleolar PELP1 complex, driving 28S rRNA maturation and regulating the pre‑60S recruitment of the remodeling ATPase MDN1. Related references: PMID:21326211
• The protein localizes dynamically to the nucleus, nucleolus, and cytoplasm, consistent with its dual roles in genomic transcription and cytoplasmic signaling.
• Post‑translational modifications reported for PELP1 include acetylation, phosphorylation, and ubiquitin‑like conjugation (SUMOylation), which likely govern its subcellular partitioning and protein–protein interactions.
• It is widely expressed across tissues and contributes to E2‑mediated cell cycle progression via physical association with the retinoblastoma protein RB1.
• PELP1 also orchestrates formation of the 5FMC complex (Five Friends of Methylated CHTOP) to control desumoylation and transactivation of ZNF148 target genes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C‑terminal region (aa 884–1130) of human PELP1, suggesting that the antibody may recognize a domain distinct from known protein–protein interaction motifs; validate the expected band at ~120 kDa in your sample system.
• For IHC‑P, consider optimizing antigen retrieval conditions (e.g., citrate buffer pH 6.0 or EDTA pH 9.0) and include appropriate tissue controls to confirm specific staining.
• In ELISA applications, the use of recombinant protein or well‑characterized cell lysates can help establish a reliable detection range; cross‑reaction with mouse and rat orthologs (predicted 90 %+ sequence identity) may allow multi‑species assays.
• Because PELP1 is subject to multiple post‑translational modifications, band shifts or multiple bands on western blot can occur; include phosphatase inhibitors during sample preparation if phosphorylation‑dependent shifts are anticipated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to deliver a comprehensive portfolio of IVD raw materials, including validated antibody pairs, polyclonal and monoclonal antibodies, and bulk ancillary reagents. For targets like PELP1—central to estrogen receptor biology, rRNA processing, and tumor cell signaling—we can source anti‑PELP1 clones, matched antigen/positive controls, and rare‑target custom material to accelerate your assay development. Whether you need a single reagent or a complete sourcing solution from concept to clinic, CamelBio bridges the gap with reliable, scalable supply.