Anti-PELI3 Polyclonal Antibody for WB, ELISA - Q8N2H9

Core Product Specifications and Parameters

Biological Background: PELI3 Function and Localization

• PELI3 is an E3 ubiquitin-protein ligase pellino homolog 3, a member of the pellino family of E3 ubiquitin ligases that catalyzes the covalent attachment of ubiquitin moieties onto substrate proteins. It plays a key role in the TLR and IL-1 signaling pathways via interaction with the IRAK-TRAF6 complex. Related references: PMID:12874243, PMID:17675297
• It mediates 'Lys-63'-linked polyubiquitination of IRAK1 and can activate the transcription factors AP1/JUN and ELK1, linking innate immune receptor activation to downstream gene expression. Related references: PMID:12874243
• PELI3 acts as a regulator of innate immunity by mediating 'Lys-63'-linked polyubiquitination of RIPK2 downstream of the NOD1 and NOD2 pathogen-recognition receptors, transforming RIPK2 into a scaffolding protein for NF-kappa-B and MAP kinases signaling (By similarity).
• Tissue expression: Highly expressed in brain, heart, and testis; lower expression in kidney, liver, lung, placenta, small intestine, spleen, and stomach. Isoform 1 is not expressed in lung.
• PELI3 is subject to alternative splicing and is classified as a phosphoprotein, transferase, and a component of the ubiquitin conjugation pathway, consistent with its role in immune signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-280) of human PELI3. The resulting polyclonal antibody may detect multiple isoforms if the epitope is conserved across variants.
• For Western blot, consider using reduced and denatured samples with appropriate molecular weight markers; the predicted molecular weight is approximately 51 kDa.
• Optimal working dilutions for WB and ELISA should be determined empirically using positive control lysates (e.g., from brain or testis) or recombinant PELI3 protein.
• Validate antibody specificity and performance in the relevant sample system, accounting for tissue-specific expression patterns and potential cross-reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-PELI3 polyclonal antibody is a valuable tool for innate immunity and ubiquitination pathway research. CamelBio offers bulk sourcing, validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents to streamline your assay development and rare target material acquisition.