Anti-PEF1 Rabbit Monoclonal Antibody for WB - Q9UBV8

Core Product Specifications and Parameters

Biological Background: PEF1 Function and Localization

• Peflin (PEF1) is a 30 kDa calcium-binding protein belonging to the penta-EF-hand (PEF) family, also known as PEF protein with a long N-terminal hydrophobic domain.
• It functions as a Ca²⁺-dependent adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes.
• In complex with PDCD6, PEF1 mediates ER-Golgi transport by regulating COPII coat size: the heterodimer bridges the BCR(KLHL12) complex to SEC31, promoting SEC31 monoubiquitination and collagen export (PubMed:27716508). Related references: PMID:27716508
• Conflicting evidence suggests PEF1 may also act as a negative regulator of PDCD6; some studies show calcium-induced dissociation of PEF1 from PDCD6, possibly inhibiting PDCD6-SEC31 interaction (PubMed:11278427). Related references: PMID:11278427
• Subcellularly, PEF1 localizes to the cytoplasm, endoplasmic reticulum, and COPII-coated vesicle membranes, consistent with its role in secretory pathway regulation.
• Key post-translational modifications and structural features include isopeptide bond formation, ubiquitin conjugation, and tandem penta-EF-hand repeats that coordinate calcium binding.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 50–150 of human PEF1 (Q9UBV8). Confirm target identity by peptide competition or knockout validation in the relevant sample system.
• For Western blot, expect a band at ~30 kDa (calculated molecular weight 30.4 kDa) and validate detection in human, mouse, or rat lysates.
• For ELISA, consider using this antibody as part of a matched pair or for direct antigen detection; titrate in the assay buffer of choice.
• Note that PEF1 function is calcium-dependent; for functional assays, include appropriate Ca²⁺ conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutions streamlined access to high-quality IVD raw materials. This anti-PEF1 monoclonal antibody supports studies of ER-Golgi transport and calcium signaling. Beyond this product, CamelBio provides validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development and biomarker research.