Anti-PEDF/SERPINF1 Rabbit Polyclonal Antibody for WB, ELISA - P36955

Core Product Specifications and Parameters

Biological Background: PEDF/SERPINF1 Function and Localization

• Encoded by the SERPINF1 gene, pigment epithelium-derived factor (PEDF) is a secreted glycoprotein belonging to the serine protease inhibitor (serpin) family, yet it lacks protease inhibitory activity due to its inability to undergo the typical stressed-to-relaxed conformational change.
• PEDF functions as a potent neurotrophic factor, promoting extensive neuronal differentiation in retinoblastoma cells, and as an endogenous inhibitor of angiogenesis.
• The protein is primarily secreted and localizes to melanosomes, with high expression in retinal pigment epithelial cells and blood plasma.
• Post-translational modifications include N-linked glycosylation, phosphorylation, and pyroglutamic acid formation, contributing to its functional regulation.
• Mutations in SERPINF1 are associated with osteogenesis imperfecta and dwarfism, highlighting its role in bone development and growth.
• Keywords from UniProt: 3D-structure, Direct protein sequencing, Dwarfism, Glycoprotein, Osteogenesis imperfecta, Phosphoprotein, Proteomics identification, Pyrrolidone carboxylic acid, Secreted, Signal.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a synthetic peptide spanning amino acids 141-240 of human PEDF/SERPINF1; it is recommended for Western blot (WB) and ELISA applications.
• Given the cross-reactivity with mouse and rat, consider validating detection in retinal pigment epithelial cells or plasma samples from these species.
• For WB, the expected protein weight is approximately 46 kDa; include appropriate positive and negative controls to confirm specificity.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw materials, providing access to this anti-PEDF/SERPINF1 polyclonal antibody alongside a broad portfolio of validated antibodies, recombinant proteins, and ancillary reagents. Whether you need bulk polyclonal antibodies, matched antibody pairs, or custom sourcing for rare targets in angiogenesis and neurobiology, we support your diagnostic development from concept to clinic.