Anti-PEDF/SERPINF1 Polyclonal Antibody for WB, IF/ICC, ELISA - P36955

Core Product Specifications and Parameters

Biological Background: PEDF/SERPINF1 Function and Localization

• Pigment epithelium-derived factor (PEDF), encoded by SERPINF1, is a secreted glycoprotein that belongs to the serpin family but lacks serine protease inhibitory activity due to its inability to undergo the stressed-to-relaxed conformational transition.
• PEDF acts as a potent neurotrophic factor, capable of inducing extensive neuronal differentiation in retinoblastoma cells.
• It is also a potent endogenous inhibitor of angiogenesis, suppressing new blood vessel formation.
• The protein is primarily localized to the extracellular space upon secretion and is also found in melanosomes.
• PEDF is expressed in retinal pigment epithelial cells and is present in blood plasma.
• Alternative names include Cell proliferation-inducing gene 35 protein (EPC-1) and Serpin F1.
• Post-translational modifications include glycosylation and phosphorylation, and it contains a pyrrolidone carboxylic acid at the N-terminus.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal peptide sequence (aa 141-240), making the antibody suitable for detecting both native and denatured PEDF in applications like WB, where standard SDS-PAGE and transfer protocols can be applied. The predicted molecular weight is approximately 46 kDa.
• For IF/ICC, consider optimizing fixation and permeabilization conditions, as PEDF is a secreted protein; extracellular staining may be observed without permeabilization, while intracellular pools may require membrane permeabilization.
• The antibody shows cross-reactivity with mouse, enabling its use in mouse-derived samples and preclinical models; however, validation in the specific experimental system is recommended.
• In ELISA, confirm specificity by including appropriate controls, such as peptide competition assays, to ensure signal corresponds to the target protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive IVD raw-material sourcing platform, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies—including this PEDF/SERPINF1 polyclonal antibody—to advance angiogenesis and neurotrophic research. We supply bulk ancillary reagents and specialize in sourcing rare-target raw materials, ensuring a seamless transition from concept to clinic for diagnostic manufacturers, labs, and research institutes.