Anti-PEBP4 Rabbit Polyclonal Antibody - Q9D9G2

Core Product Specifications and Parameters

Biological Background: PEBP4 Function and Localization

• PEBP4 (Phosphatidylethanolamine-binding protein 4) is a 27 kDa protein encoded by the Pebp4 gene in mouse.
• It belongs to the phosphatidylethanolamine-binding protein (PEBP) family, which are implicated in various signaling pathways.
• PEBP4 promotes AKT phosphorylation, suggesting a potential role in the PI3K-AKT signaling cascade.
• It is classified as a secreted glycoprotein, containing a signal peptide for extracellular localization.
• As a reference proteome component, PEBP4 is considered a well-characterized protein.
• The protein may also be referred to as PEBP-4 or PEBP4.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 90-242) of mouse PEBP4, which may confer specificity for detecting the full-length protein.
• For Western blot (WB), the predicted molecular weight is ~27 kDa; use appropriate gel percentage and validate with positive controls.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider antigen retrieval and permeabilization protocols given the secreted nature of PEBP4.
• For ELISA, evaluate the antibody pair suitability by testing serial dilutions with recombinant protein or lysates.
• As the antibody shows cross-reactivity with human and rat, validate performance in these species-specific samples to ensure consistent results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research groups with end-to-end access to IVD raw materials, including validated antibodies, antigens, and ancillary reagents. For studies on the PI3K-AKT signaling pathway, we offer reliable antibody pairs and bulk polyclonal antibodies targeting PEBP4 and related proteins. Explore our one-stop procurement solutions to fast-track your assay development, from concept to clinical application.