Anti-PEBP4 Polyclonal Antibody for WB and ELISA - Q96S96

Core Product Specifications and Parameters

Biological Background: PEBP4 Function and Localization

• PEBP4, also known as cousin-of-RKIP 1 (CORK1), is a member of the phosphatidylethanolamine-binding protein (PEBP) family, which includes the metastasis suppressor RKIP/PEBP1.
• Its primary annotated function is the promotion of AKT phosphorylation, suggesting a role in the PI3K-AKT signaling pathway that regulates cell survival, proliferation, and metabolism.
• The protein is predicted to be secreted, as indicated by the presence of a signal peptide, and is annotated as a glycoprotein in curated databases.
• Subcellular localization data point to an extracellular role, distinguishing it from some other PEBP family members that localize to the cytoplasm or nucleus.
• Proteomics studies have confirmed its expression in human samples, and it is included in the reference proteome set.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 23-120 of human PEBP4, targeting the N-terminal region; mouse cross-reactivity suggests conservation of this epitope.
• In Western blotting, expect a band near 26 kDa; however, glycosylation may cause a higher apparent molecular weight shift.
• Validate antibody performance in your specific sample system; consider using positive controls from known PEBP4-expressing cell lines or tissues.
• For ELISA, optimization of coating and detection conditions is recommended; pairing with a validated secondary or detection antibody can improve sensitivity.
• As a secreted protein, analyze conditioned media for extracellular PEBP4 or assess intracellular pools under appropriate stimulation conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers comprehensive access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents for diagnostic development. Our portfolio supports research into signaling pathways such as PI3K-AKT and targets like PEBP4, a secreted factor with emerging roles in cell signaling. We also offer rare-target sourcing services, bridging critical gaps from concept to clinic for diagnostic manufacturers and labs.