Anti-PEBP1 Rabbit Monoclonal Antibody for WB, ELISA - P30086

Core Product Specifications and Parameters

Biological Background: PEBP1 Function and Localization

• PEBP1 (Phosphatidylethanolamine-binding protein 1), also known as RKIP, HCNP, and PBP, is a 21 kDa cytosolic protein belonging to the phosphatidylethanolamine-binding protein family. It is encoded by the PEBP1 gene on chromosome 12q24.23.
• The protein binds ATP, opioids, and phosphatidylethanolamine with relatively high affinity, and exhibits lower affinity for phosphatidylinositol and phosphatidylcholine.
• It acts as a serine protease inhibitor, suppressing the activity of thrombin, neuropsin, and chymotrypsin, while not affecting trypsin, tissue-type plasminogen activator, or elastase (by similarity).
• PEBP1 serves as a key negative regulator of the RAF/MEK/ERK signaling cascade by binding to RAF1, disrupting the RAF1-MEK complex, and competitively inhibiting MEK phosphorylation.
• As the precursor of hippocampal cholinergic neurostimulating peptide (HCNP), it enhances choline acetyltransferase production in presynaptic cholinergic neurons, potentially through a specific receptor-mediated mechanism (by similarity).
• The protein undergoes phosphorylation and contains an ATP-binding site; it is localized predominantly in the cytoplasm and has been identified in high-throughput proteomics analyses.
• UniProt lists a molecular weight of 21,057 Da, consistent with the observed 21 kDa band; intramolecular disulfide bonds contribute to its structural stability.

Experimental Guidance and Technical Tips

• The synthetic immunogen (aa 1-100) is designed to be specific to the N-terminal region; confirm specificity by peptide competition assay and sequence alignment across target species.
• For western blotting, a band near 21 kDa is expected in human, mouse, and rat samples; use appropriate positive and negative controls to verify performance.
• Optimize antibody dilutions for individual assay formats; note that recommended starting dilutions from supplier are typical guidelines and should be validated in your specific experimental system.
• When using this antibody in ELISA, consider pairing with a detection antibody that recognizes a distinct epitope to avoid competition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions one-stop access to IVD raw materials, supported by technical and regulatory consulting from concept to commercialization. This anti-PEBP1 (RKIP/HCNP) rabbit monoclonal antibody is suited for investigations into MAPK signaling, neurobiology, and cancer biomarker development. Beyond single-antibody supply, we provide validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets to streamline your assay development workflow.