Anti-PEBP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P30086

Core Product Specifications and Parameters

Biological Background: PEBP1 Function and Localization

• PEBP1, also known as Raf kinase inhibitor protein (RKIP) and hippocampal cholinergic neurostimulating peptide (HCNP), is encoded by the PEBP1 gene (also designated PBP). It belongs to the phosphatidylethanolamine-binding protein family and has a molecular weight of approximately 21 kDa.
• The protein functions as a serine protease inhibitor, inhibiting thrombin, neuropsin, and chymotrypsin, while also binding ATP, opioids, and phosphatidylethanolamine with lower affinity for other phospholipids.
• A key role of PEBP1 is negative regulation of the RAF/MEK/ERK pathway: it inhibits RAF1 kinase activity by preventing its activation, dissociating the RAF1/MEK complex, and competitively inhibiting MEK phosphorylation.
• The HCNP precursor is processed to yield the neuropeptide HCNP, which enhances choline acetyltransferase production in presynaptic cholinergic neurons without affecting acetylcholinesterase, likely acting through a specific receptor.
• Subcellularly, PEBP1 localizes to the cytoplasm.
• Post-translational modifications include phosphorylation (indicated by the keyword 'Phosphoprotein') and a disulfide bond, which may regulate its activity and stability.
• Tissue-specifically, the HCNP activity is associated with the central nervous system, highlighting its role in neurobiology.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 1–187, covering nearly the full-length human PEBP1. This suggests the polyclonal antibody may recognize epitopes across the entire protein sequence.
• For Western blotting, a single band around 21 kDa is expected in human, mouse, or rat cell or tissue lysates. Use appropriate loading controls and validate specificity in knockout or knockdown samples.
• In immunofluorescence/ICC, anticipate cytoplasmic staining. Fixation and permeabilization conditions should be optimized for each cell type.
• For immunoprecipitation, consider the antibody’s compatibility with native or denatured protein conditions. Pilot experiments with positive and negative controls are recommended.
• Due to the polyclonal nature, lot-to-lot consistency should be verified when transitioning between large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, contract research organizations, and academic labs with streamlined access to high-quality IVD raw materials and technical support. This anti-PEBP1 polyclonal antibody targets a multifunctional protein implicated in kinase signaling, neurobiology, and cancer, making it a valuable tool for biomarker discovery and assay development. Beyond individual antibodies, CamelBio can supply validated antibody pairs, recombinant proteins, bulk ancillary reagents, and customized sourcing solutions for rare targets—bridging your project from concept to clinic.