Anti-PDZK1 Rabbit Polyclonal Antibody for WB, ELISA - Q5T2W1

Core Product Specifications and Parameters

Biological Background: PDZK1 Function and Localization

• PDZK1 (also known as NHERF3, CAP70, or NaPi-Cap1) is a PDZ domain-containing scaffolding protein that belongs to the Na(+)/H(+) exchange regulatory factor family.
• It serves as a scaffold connecting plasma membrane proteins and regulatory components, regulating their surface expression in the apical domains of epithelial cells.
• PDZK1 coordinates diverse regulatory processes for ion transport and second messenger cascades; in complex with NHERF1, it clusters functionally dependent proteins to modulate trafficking and activity.
• It plays a role in multidrug resistance through interaction with ABCC2 and PDZK1IP1, and potentiates the chloride channel activity of CFTR.
• PDZK1 is required for normal cell-surface expression of SCARB1, thereby helping to maintain plasma cholesterol levels, and it ensures proper localization and function of the chloride-anion exchanger SLC26A6 in the kidney proximal tubule.
• The protein localizes to the cell membrane and is expressed specifically in epithelial cells of the kidney (brush border of proximal tubule), pancreas, liver, and small intestine, with lower levels in the adrenal cortex, testis, and stomach; it is overexpressed in several carcinomas.
• PDZK1 is a phosphoprotein and may exist in multiple isoforms due to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-260 of human PDZK1, covering the N-terminal region rich in PDZ domains. This design may recognize multiple isoforms and supports the observed mouse cross-reactivity.
• For Western blot, expect a band at approximately 57 kDa; consider that additional bands may appear due to splice variants or post-translational modifications.
• Validate the antibody in the relevant cell or tissue lysate, such as kidney, pancreas, or liver epithelial cells, and optimize dilutions for your specific experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-PDZK1 polyclonal antibody, along with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, is available to support your epithelial biology and ion transport research. Let CamelBio streamline your supply chain and accelerate your diagnostic assay development.