Anti-PDXP Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q96GD0

Core Product Specifications and Parameters

Biological Background: PDXP (Chronophin) Function and Localization

• PDXP (chronophin) is a pyridoxal phosphate (PLP) phosphatase, also known as PLP phosphatase or CIN, and belongs to the haloacid dehalogenase (HAD) superfamily of hydrolases. Related references: PMID:14522954
• Functions as a key regulator of vitamin B6 metabolism by dephosphorylating PLP, pyridoxine 5'-phosphate (PNP), and pyridoxamine 5'-phosphate (PMP), with substrate preference PLP > PNP > PMP. Related references: PMID:14522954, PMID:8132548
• Acts as a protein serine phosphatase that specifically dephosphorylates Ser-3 in cofilin and other ADF/cofilin family members, thereby regulating actin cytoskeleton dynamics, mitosis, and cytokinesis. Does not dephosphorylate LIMK1 or phosphotyrosine. Related references: PMID:15580268
• Subcellular localization includes cytoplasm, cytosol, cytoskeleton, and cell projections such as ruffles and lamellipodia, as well as transient membrane association.
• Ubiquitously expressed at the protein level, with high expression in the central nervous system, liver, and testis; fetal expression is weak except in the brain. Related references: PMID:23223568, PMID:14522954, PMID:15580268
• Requires magnesium ions for catalytic activity and is subject to alternative initiation and alternative splicing, generating multiple isoforms.
• Keywords: hydrolase, magnesium, metal-binding, pyridoxal phosphate, reference proteome, and direct protein sequencing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 200 to end) of human PDXP; sequence alignment across species should be verified if cross-reactivity beyond human, mouse, and rat is required.
• For Western blot, a band near 32 kDa is expected, but alternative splicing or post-translational modifications may produce additional bands; validate in relevant lysates (e.g., brain, liver, or testis tissue).
• In immunohistochemistry, consider the ubiquitous but enriched expression in CNS tissues, and optimize antigen retrieval conditions empirically.
• For immunofluorescence/ICC, anticipate cytosolic and cytoskeletal staining with possible enrichment at membrane ruffles and lamellipodia; use appropriate fixation and permeabilization protocols.
• When used in ELISA, the antibody can serve as a capture or detection reagent; however, pairing with a matched antibody and testing in the relevant matrix is recommended.
• No validated working dilutions are provided; determine optimal concentrations by titration in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic developers with validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-PDXP (chronophin) polyclonal antibody supports research into vitamin B6 metabolism, cofilin signaling, and neuronal development. Whether you need rare-target antibodies or scalable assay components, CamelBio streamlines procurement from concept to clinic.