Anti-PDXK Polyclonal Antibody for WB, IF/ICC, ELISA - O00764

Core Product Specifications and Parameters

Biological Background: PDXK Function and Localization

• PDXK (Pyridoxal kinase), also known as pyridoxine kinase, is a key enzyme in the vitamin B6 salvage pathway, belonging to the pyridoxal kinase family.
• Catalyzes the ATP-dependent phosphorylation of dietary vitamin B6 vitamers (pyridoxal, pyridoxine, pyridoxamine) to their respective 5'-phosphates, generating the active cofactor pyridoxal 5'-phosphate (PLP). Related references: PMID:10987144 PMID:17766369 PMID:19351586
• The PDXK gene is located on chromosome 21 and is ubiquitously expressed, with highest levels detected in testis and spermatozoa. Related references: PMID:31187503 PMID:9099727
• Subcellularly, PDXK predominantly localizes to the cytoplasm and cytosol, consistent with its role in vitamin B6 metabolism.
• PDXK activity is dependent on divalent metal ions such as magnesium, manganese, zinc, and cobalt, as indicated by its metal-binding properties.
• Mutations in PDXK are associated with Charcot-Marie-Tooth disease type 6C (CMT6C) and other forms of neuropathy, highlighting its role in neuronal health.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomics approaches.
• Alternative splicing produces multiple transcript variants, potentially modulating enzyme activity or localization.

Experimental Guidance and Technical Tips

• As this polyclonal antibody was raised against full-length recombinant human PDXK, it may detect multiple epitopes and could exhibit higher sensitivity in applications like Western blot, but consider potential cross-reactivity with other species or isoforms.
• For Western blot (WB) under reducing conditions, the predicted molecular weight of PDXK is ~35 kDa; however, validate using appropriate positive controls such as human, mouse, or rat cell lysates known to express PDXK.
• For immunofluorescence/immunocytochemistry (IF/ICC), fixation and permeabilization conditions should be optimized to preserve the target's cytoplasmic localization; include a negative control (no primary antibody) to assess background.
• When using ELISA, pair with a suitable capture or detection antibody and confirm specificity with recombinant PDXK protein if available.
• Given the antibody's broad species reactivity (human, mouse, rat), it is suitable for cross-species studies; nevertheless, confirm reactivity in your specific model system before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw-material procurement by offering validated antibodies, biological reagents, and technical services from discovery through clinical development. For PDXK-related studies—whether you need antibody pairs, bulk polyclonal antibodies, or specialized reagents for vitamin B6 metabolism research—CamelBio ensures reliable supply and expert support. Contact us to source rare targets or customize raw materials for your diagnostic development needs.