Anti-PDXK Monoclonal Antibody for WB, ELISA - O00764

Core Product Specifications and Parameters

Biological Background: PDXK Function and Localization

• PDXK encodes pyridoxal kinase, a member of the kinase/transferase family that phosphorylates vitamin B6 vitamers (pyridoxal, pyridoxine, pyridoxamine) to their active 5'-phosphate forms (PLP, PNP, PMP). Related references: PMID:10987144 PMID:17766369 PMID:19351586
• PLP, the major active coenzyme, serves as a cofactor for over 140 enzymes, including those involved in amino acid metabolism, neurotransmitter synthesis, and glycogen breakdown.
• The protein localizes predominantly to the cytoplasm and cytosol.
• Ubiquitously expressed with highest levels in testis. Related references: PMID:31187503 PMID:9099727
• The kinase activity requires divalent metal ions such as Mg²⁺, Mn²⁺, Co²⁺, or Zn²⁺ for catalysis.
• Post‑translational modifications include acetylation and phosphorylation; alternative splicing generates multiple isoforms.
• Mutations in PDXK have been associated with Charcot‑Marie‑Tooth disease and other peripheral neuropathies.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is approximately 35 kDa; consider using a reducing SDS-PAGE system and validate with a cell line or tissue known to express PDXK, such as testis.
• For ELISA, the antibody can be used as a capture or detection reagent; titrate with recombinant PDXK protein to determine optimal working concentration.
• As the immunogen corresponds to the full-length human sequence, cross-reactivity with orthologs from other species is not guaranteed; species-specific validation may be required.
• The antibody may detect multiple isoforms due to alternative splicing; confirm band pattern in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and labs from concept to clinic. This PDXK antibody supports research into vitamin B6 metabolism and kinase-related pathways, complementing our portfolio of validated monoclonal antibodies, bulk ancillary reagents, and custom sourcing services for rare and emerging targets. We streamline procurement so you can accelerate assay development and scale with confidence.