Anti-PDX1 Polyclonal Antibody for WB and ELISA - P52945

Core Product Specifications and Parameters

Biological Background: PDX1 Function and Localization

• PDX1 (Pancreas/duodenum homeobox protein 1), also known as IPF1, IUF1, IDX-1, STF-1, GSF, is a homeobox transcription factor essential for pancreatic development and beta-cell function.
• Core transcriptional activator of insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2 genes; critically involved in glucose-dependent regulation of insulin gene transcription.
• In pancreatic acinar cells, forms a complex with PBX1b and MEIS2b to activate the ELA1 enhancer, cooperating with the PTF1 complex bound to the enhancer A element.
• Binds preferentially to the DNA motif 5'-[CT]TAAT[TG]-3'.
• During embryonic development, specifies the early pancreatic epithelium, promoting proliferation, branching, and differentiation; in adults, maintains the hormone-producing phenotype of beta-cells.
• Subcellular localization is primarily nuclear, but also detected in the cytoplasm (cytosol).
• Tissue expression is restricted to duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, with low levels in acinar cells).
• The theoretical molecular weight is approximately 30.8 kDa; on SDS-PAGE, the protein typically migrates around 31 kDa.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic peptide spanning amino acids 1-100 of human PDX1 (NP_000200.1). The sequence is highly conserved between human and mouse, consistent with its cross-reactivity.
• For Western blot, use whole cell lysates from human or mouse pancreatic cell lines (e.g., MIN6, β-TC-6) or primary islets as a positive control. Expect a specific band at approximately 31 kDa.
• For ELISA, optimize coating buffer and antibody concentration for your specific assay format; the antibody may be used for direct or indirect detection.
• PDX1 expression is developmentally regulated and tissue-restricted; always include appropriate positive and negative controls, especially when using non-pancreatic samples.
• To prevent proteolytic degradation, keep samples chilled and use protease inhibitors during lysis.
• Validate the antibody in your own experimental system, as performance may vary with sample preparation and detection methods.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing high-quality IVD raw materials and comprehensive sourcing solutions for diagnostic manufacturers, research laboratories, and academic institutes. For targets like PDX1—a key transcription factor in pancreatic development and diabetes—CamelBio can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assist with rare target raw-material procurement. Whether you need consistent lot-to-lot performance or customized reagent sourcing, CamelBio supports your project from concept to clinic.