Anti-PDP2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9P2J9

Core Product Specifications and Parameters

Biological Background: PDP2 Function and Localization

• PDP2, also known as PPM2B or PDPC2, is a mitochondrial protein phosphatase belonging to the PP2C family of serine/threonine phosphatases.
• It catalyzes the dephosphorylation of the alpha subunit of the pyruvate dehydrogenase complex (PDC), reactivating the complex to convert pyruvate into acetyl-CoA, a critical step in cellular energy metabolism.
• Its activity requires magnesium or manganese ions as cofactors, consistent with its metal-binding properties.
• PDP2 is localized exclusively in the mitochondria, where it regulates glucose oxidation through PDC modulation.
• The protein plays a pivotal role in T cell metabolism, particularly in controlling the metabolic switch and function of T-helper 17 (Th17) cells.
• As a key phosphatase, PDP2 is implicated in immunometabolism and may influence inflammation and autoimmunity through its regulatory effects on T cells.
• The UniProt record classifies PDP2 as a hydrolase and protein phosphatase, with a transit peptide directing it to mitochondria.

Experimental Guidance and Technical Tips

• The immunogen covers a central region of PDP2 (amino acids 67–250), which is conserved in mouse and rat, supporting cross-reactivity assessment.
• For Western blot, a band at approximately 60 kDa is expected. Consider optimizing lysis buffers and mitochondrial enrichment if endogenous levels are low.
• In immunofluorescence, mitochondrial staining patterns should be confirmed with organelle markers.
• ELISA applications may be suitable for detecting PDP2 in purified protein or cell lysates; validate the assay range in your sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop partner for IVD raw materials, offering validated antibodies like this anti-PDP2 polyclonal for metabolism and immunology research. We provide optimized antibody pairs, bulk ancillary reagents, and sourcing support for rare targets to streamline your diagnostic development from concept to clinic.