Anti-PDP1 Polyclonal Antibody for WB - Q9P0J1

Core Product Specifications and Parameters

Biological Background: PDP1 Function and Localization

• PDP1 is a mitochondrial enzyme that dephosphorylates and reactivates the alpha subunit of the E1 component of the pyruvate dehydrogenase complex (PDC), stimulating conversion of pyruvate into acetyl-CoA and linking glycolysis to the citric acid cycle.
• Also known as protein phosphatase 2C (PPM2C), it belongs to the PP2C family of Ser/Thr phosphatases and requires divalent metal ions such as magnesium or manganese for activity.
• Alternative splicing produces multiple isoforms, which may differ in regulation or localization.
• The enzyme localizes primarily to the mitochondrion, where it accesses its substrate in the PDC.
• Post-translational modifications include acetylation, which may influence enzyme function.
• Mutations in PDP1 are associated with disease variants, underscoring its importance in metabolic regulation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 72–260 of human PDP1, a central region conserved across isoforms; consider validating isoform recognition in your specific sample system.
• For western blotting, the predicted molecular weight is ~61 kDa; run appropriate molecular weight markers and consider using a mitochondrial fraction for enrichment.
• In immunofluorescence/ICC, expect a mitochondrial staining pattern; co-stain with a mitochondrial marker to confirm localization.
• For ELISA, this polyclonal antibody can be used as a capture or detection reagent; optimize coating concentrations and blocking conditions for your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents for metabolic disease panels. This PDP1 antibody supports pyruvate dehydrogenase and mitochondrial function research, and our team can assist with rare-target raw-material sourcing to accelerate your development from concept to clinic.