Anti-PDLIM5 Rabbit Polyclonal Antibody for WB, ELISA - Q96HC4

Core Product Specifications and Parameters

Biological Background: PDLIM5 Function and Localization

• PDLIM5 (PDZ and LIM domain protein 5), also known as Enigma homolog (ENH), ENH1, L9, and LIM, is a member of the PDZ-LIM protein family characterized by PDZ and LIM domains. It exists as multiple isoforms generated by alternative splicing, with isoform 1 containing LIM domains and isoform 2 lacking them.
• In the heart, isoform 1 scaffolds protein kinase C (PKC) to the Z-disk region, playing a crucial role in heart development and the regulation of cardiomyocyte expansion. Overexpression of PDLIM5 promotes cardiac hypertrophy.
• Isoforms lacking the LIM domains, such as isoform 2, may negatively modulate the scaffolding activity of isoform 1, suggesting a regulatory balance in cardiac function.
• In neurons, PDLIM5 contributes to the regulation of dendritic spine morphogenesis. Isoform 1 expression favors spine thinning and elongation, while the protein overall is required to restrain excitatory synapse growth.
• Subcellularly, PDLIM5 localizes to the postsynaptic density, presynaptic compartments, and cytoplasm (cytosol), consistent with roles in synaptic organization and intracellular signaling.
• Tissue specificity is prominent in heart and skeletal muscle, but expression is also commonly increased in the brain of patients with bipolar disorder, schizophrenia, and major depression.
• The protein contains LIM domains that coordinate zinc ions (metal-binding) and is subject to various post-translational modifications including acetylation, phosphorylation, and ubiquitination (isopeptide bond formation).
• Keywords associated with PDLIM5 include: 3D-structure, Alternative splicing, Cell projection, Cytoplasm, LIM domain, Metal-binding, Phosphoprotein, Synapse, Ubl conjugation, and Zinc, highlighting its structural and functional versatility.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 300–400 of human PDLIM5 (NP_006448.5). This region is located between the PDZ and LIM domains; consider that the antibody may recognize both isoforms if the epitope is conserved. Validate isoform specificity if required for your study.
• For Western blot, PDLIM5 has a predicted molecular weight of approximately 64 kDa. Use appropriate protein markers and consider starting with a dilution range like 1:500–1:2000, optimizing based on signal and background. Positive controls can include human heart or brain tissue lysates.
• For ELISA, the antibody can be used for target capture or detection. Pair with an appropriate enzyme-conjugated secondary antibody and validate with recombinant protein or biological samples.
• When working with human samples, be mindful of expression patterns: higher expression in heart and skeletal muscle, and increased in brain under certain pathological conditions. Include relevant controls to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a streamlined one-stop platform for IVD raw-material sourcing, technical services, and consulting—from concept to clinic. This anti-PDLIM5 polyclonal antibody supports research into cardiac hypertrophy signaling and synaptic plasticity mechanisms. Complement your procurement with CamelBio’s validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and expert assistance in sourcing rare targets.