Anti-PDLIM1/CLP36 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - O00151

Core Product Specifications and Parameters

Biological Background: PDLIM1/CLP36 Function and Localization

• PDLIM1 (PDZ and LIM domain protein 1), also known as CLP-36, elfin, or CLIM1, is a 36 kDa cytoskeletal adapter protein.
• It acts as a molecular scaffold that recruits signaling proteins, including kinases, to the actin cytoskeleton, thereby regulating cytoskeletal organization and signal transduction. Related references: PMID:10861853
• PDLIM1 is essential for stress fiber assembly, disassembly, and directional remodeling in fibroblasts.
• It mediates the recruitment of α-actinin-1 (ACTN1) and palladin (PALLD) to stress fibers, which are critical for fiber stability and contractility.
• PDLIM1 is required for fibroblast migration and maintenance of cell polarity, influencing wound healing and tissue repair processes.
• Subcellularly, PDLIM1 localizes to the cytoplasm, actin cytoskeleton, and sarcomeric Z-lines in myofibrils, suggesting roles in both non-muscle and muscle cells.
• Tissue distribution is highest in heart and skeletal muscle, moderate in spleen, small intestine, colon, placenta, and lung, lower in liver, thymus, kidney, prostate, and pancreas, and absent in brain, testis, ovary, and peripheral blood leukocytes.
• PDLIM1 contains a PDZ domain and a LIM domain; the LIM domain coordinates zinc and mediates protein–protein interactions, while post-translational modifications include acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 200–300) of human PDLIM1, which may confer target specificity; verify reactivity in your experimental system.
• For Western blotting, a starting antibody dilution is typically in the range of 1:500–1:2000, but optimal dilutions should be determined empirically.
• In immunofluorescence/ICC, PDLIM1 is expected to co-localize with actin stress fibers; include appropriate positive controls (e.g., fibroblasts) and counterstain filamentous actin (phalloidin) to confirm localization.
• ELISA performance can be affected by coating conditions and detection reagents; consider pairing with a validated secondary antibody and standard curve.
• Cross-reactivity with mouse suggests suitability for rodent models; however, perform species-specific validation.
• PDLIM1 expression level varies by tissue; select samples that express the target (e.g., skeletal muscle, heart) for robust detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop channel for sourcing high-quality IVD raw materials. This PDLIM1/CLP36 antibody, targeting a key cytoskeletal adapter implicated in cell migration and mechanotransduction, exemplifies our capability to offer validated monoclonal antibodies, matched antibody pairs, and bulk ancillary reagents for diagnostic and research applications. By bridging reliable supply with technical insights, CamelBio accelerates your development from concept to clinic.