Anti-PDK3 Polyclonal Antibody for WB, ELISA - Q15120

Core Product Specifications and Parameters

Biological Background: PDK3 Function and Localization

• PDK3 encodes a mitochondrial pyruvate dehydrogenase kinase that inhibits the pyruvate dehydrogenase complex through phosphorylation of the E1 alpha subunits PDHA1 and PDHA2, thereby controlling the entry of pyruvate into the tricarboxylic acid cycle.
• This kinase acts as a critical metabolic switch, reducing glucose utilization and promoting fatty acid oxidation in response to prolonged fasting or a high-fat diet, which helps maintain glucose homeostasis.
• PDK3 is one of four PDK isoforms (PDK1-4) that differentially regulate the pyruvate dehydrogenase complex across various tissues and metabolic states.
• The PDK3 preprotein is targeted to the mitochondrial matrix, where it exerts its regulatory function on the pyruvate dehydrogenase complex.
• PDK3 is an ATP-binding kinase that belongs to the transferase family; it may also contribute to the generation of reactive oxygen species.
• Tissue expression is notably high in heart, skeletal muscle, spinal cord, and both fetal and adult brain, consistent with its role in modulating metabolism in highly oxidative tissues.
• Pathogenic variants in PDK3 are associated with Charcot-Marie-Tooth disease, a peripheral neuropathy, highlighting its importance in neuronal function.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 137-406 of human PDK3, covering a substantial portion of the kinase domain; this design may recognize both full-length and possible alternatively spliced isoforms.
• For Western blot, consider using protein lysates from tissues with high PDK3 expression such as heart, skeletal muscle, or brain; optimize antibody dilution based on preliminary experiments.
• In ELISA, evaluate direct or indirect protocols with recombinant PDK3 protein or cell lysates to confirm binding specificity.
• Given the predicted molecular weight of ~47 kDa, ensure adequate gel separation and verify band identity with appropriate positive and negative controls.
• Cross-reactivity with mouse and rat PDK3 is reported; validate in the relevant sample system if working across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering antibodies like this PDK3 polyclonal antibody for metabolic and neurological disease research. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can facilitate the sourcing of rare target raw materials. Whether you are developing assays for glucose metabolism or neuropathy, CamelBio supports your project from concept to clinic with tailored procurement solutions.