Anti-PDK3 Polyclonal Antibody for WB, ELISA - Q15120

Core Product Specifications and Parameters

Biological Background: PDK3 Function and Localization

• PDK3 encodes a mitochondrial pyruvate dehydrogenase kinase that inhibits the pyruvate dehydrogenase complex by phosphorylating the E1 subunit PDHA1, and can also phosphorylate PDHA2, thus regulating glucose metabolism and aerobic respiration.
• In response to prolonged fasting or a high-fat diet, PDK3 decreases glucose utilization and increases fat metabolism, playing a key role in metabolic adaptation and glucose homeostasis.
• PDK3 is involved in the generation of reactive oxygen species and helps maintain normal blood glucose levels under varying nutrient conditions and starvation.
• The kinase localizes to the mitochondrial matrix, consistent with its role in modulating the pyruvate dehydrogenase complex within the mitochondrion.
• PDK3 is expressed in heart, skeletal muscle, spinal cord, and both fetal and adult brain, indicating tissue-specific metabolic regulation.
• Alternative splicing of PDK3 produces multiple transcript variants, and mutations in this gene are associated with Charcot-Marie-Tooth disease type X6 (CMTX6), a neurodegenerative disorder.
• The protein possesses ATP-binding and nucleotide-binding domains essential for its kinase activity, and is classified as a transferase with a transit peptide for mitochondrial import.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole-cell lysates from human cell lines known to express PDK3 (e.g., HeLa, HepG2, or skeletal muscle-derived cells). A band at approximately 47 kDa is expected. Optimize blocking and antibody dilution based on your specific sample.
• In ELISA, the recombinant immunogen (aa 137-406) or native PDK3 from human tissue/cell extracts can be used as coating antigen. Validate linearity and sensitivity with a standard curve in the relevant biological matrix.
• Given the human-specific reactivity, this antibody is suitable for human sample analysis. Cross-reactivity with other species has not been determined; validate if used in non-human systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-PDK3 polyclonal antibody facilitates research into metabolic disorders, mitochondrial function, and Charcot-Marie-Tooth disease. For rare target sourcing or custom antibody development, our team provides end-to-end procurement and technical consulting to accelerate your project from concept to clinic.