Anti-PDIA6 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q15084

Core Product Specifications and Parameters

Biological Background: PDIA6 Function and Localization

• Protein disulfide-isomerase A6 (PDIA6), also known as ERp5 or P5, is a member of the protein disulfide isomerase family, characterized by thioredoxin-like domains and a redox-active center.
• It functions as a molecular chaperone, inhibiting aggregation of misfolded proteins in the endoplasmic reticulum (PubMed:12204115).
  Related references:
  PMID:12204115
• PDIA6 negatively regulates the unfolded protein response (UPR) by binding to UPR sensors ERN1 (IRE1) and possibly EIF2AK3 (PERK), thereby inactivating their signaling pathways (PubMed:24508390).
  Related references:
  PMID:24508390
• Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin (PubMed:15466936).
  Related references:
  PMID:15466936
• Subcellular localization includes the endoplasmic reticulum lumen, cell membrane, and melanosome, indicating diverse functional roles.
• Expressed in platelets at the protein level, consistent with its role in platelet function.
• Alternative splicing leads to multiple transcript variants, which may have distinct functional properties. Keywords: chaperone, isomerase, endoplasmic reticulum, redox-active center, phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20-300 of human PDIA6, which includes key functional domains; the resulting polyclonal antibody may recognize multiple isoforms due to alternative splicing.
• For Western blot (WB), a band at approximately 48 kDa is expected; validate using positive control lysates from human, mouse, or rat tissues or cell lines known to express PDIA6.
• For immunohistochemistry (IHC-P), consider optimizing antigen retrieval and antibody dilution to achieve specific staining in platelet-rich or ER-rich tissues.
• Cross-reactivity with mouse and rat broadens utility; however, performance should be verified in the specific experimental system.
• As a polyclonal antibody, lot-to-lot consistency is recommended to be monitored; pre-adsorption may reduce background if necessary.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio offers diagnostic manufacturers and researchers a consolidated sourcing platform for IVD raw materials, including validated antibodies like this anti-PDIA6 polyclonal antibody, ideal for studies in ER stress, platelet biology, and chaperone function.
• Beyond antibodies, we provide optimized ancillary reagents and bulk supply solutions, streamlining procurement from concept to clinical application.
• For rare or custom targets, our team facilitates sourcing and technical consulting, ensuring reliable, high-quality materials to accelerate your diagnostic development.