Anti-PDIA4 Rabbit Polyclonal Antibody - P13667

Core Product Specifications and Parameters

Biological Background: PDIA4 Function and Localization

• Protein disulfide-isomerase A4 (PDIA4), also designated ERP70/ERP72, is a member of the protein disulfide isomerase (PDI) family.
• It resides primarily in the endoplasmic reticulum lumen and has also been detected in melanosomes.
• The protein is characterized by thioredoxin-like domains and redox-active CXXC motifs, consistent with its classification as an isomerase and oxidoreductase.
• As a PDI family enzyme, PDIA4 is thought to catalyze the formation, isomerization, and reduction of disulfide bonds during oxidative protein folding.
• Keywords associated with PDIA4 include acetylation, direct protein sequencing, disulfide bond, host-virus interaction, and signal peptide, indicating a broad functional landscape.
• It is part of the human reference proteome and has been extensively validated by proteomics identification.

Experimental Guidance and Technical Tips

• For western blot, consider using whole-cell lysates from human, mouse, or rat tissues, as the antibody shows cross-reactivity; ER-enriched fractions may enhance detection.
• IHC-P and IF/ICC protocols may require antigen retrieval due to the epitope’s location within the ER lumen; permeabilization steps are critical.
• ELISA performance can be optimized by standard checkerboard titration of both coating antigen and antibody.
• This rabbit polyclonal antibody detects a band at approximately 73 kDa; validate with appropriate molecular weight markers and consider using a blocking buffer suitable for reduced and denatured samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For PDIA4 and broader ER stress-related targets, we can connect you with rare-target raw materials and expert consulting to streamline your diagnostic development from concept to clinic.