Anti-PDIA2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q13087

Core Product Specifications and Parameters

Biological Background: PDIA2 Function and Localization

• PDIA2, also known as pancreas-specific protein disulfide isomerase (PDIp), is a member of the protein disulfide isomerase family, characterized by thioredoxin-like domains and a redox-active center.
• It functions as an intracellular estrogen-binding protein, potentially modulating cellular estrogen levels and biological functions in the pancreas.
• PDIA2 may act as a molecular chaperone, inhibiting aggregation of misfolded proteins, consistent with its residence in the endoplasmic reticulum.
• The protein localizes to the endoplasmic reticulum lumen, where it likely participates in disulfide bond formation and isomerization.
• At the protein level, PDIA2 is highly expressed in the pancreas, supporting its tissue-specific role.
• Keywords include alternative splicing, glycoprotein, and signal peptide, indicating post-translational modifications and secretory pathway targeting.

Experimental Guidance and Technical Tips

• Since the immunogen corresponds to amino acids 326-525 of human PDIA2, this antibody may recognize the C-terminal region; consider validating reactivity in your target species (human, mouse, rat) with appropriate controls.
• Recommended for Western blot (WB) at typical dilutions (e.g., 1:500-1:2000) using whole-cell or tissue lysates; the observed band may be around 58 kDa.
• Suitable for immunofluorescence/immunocytochemistry (IF/ICC) to study ER localization; fixation and permeabilization methods should be optimized to preserve intracellular structures.
• For ELISA applications, consider indirect ELISA using recombinant PDIA2 protein as the coating antigen; the antibody may serve as a detection reagent.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing bridge for IVD raw materials, technical services, and consulting—from concept to clinic. We support your PDIA2 research with validated antibody pairs, bulk ancillary reagents, and optimized polyclonal antibodies for ER chaperone and estrogen-binding studies. Whether you need rare target reagents or custom formulation, our supply network ensures rapid, reliable access to critical raw materials.