Anti-PDHA1 Monoclonal Antibody for WB, IF/ICC, ELISA - P08559

Core Product Specifications and Parameters

Biological Background: PDHA1 Function and Localization

• PDHA1 encodes the alpha subunit (E1) of the pyruvate dehydrogenase complex, a mitochondrial enzyme that converts pyruvate to acetyl-CoA, linking glycolysis to the TCA cycle. Related references: PMID:17474719, PMID:19081061, PMID:7782287
• Together with PDHB, it forms the heterotetrameric E1 component, which catalyzes the thiamine pyrophosphate-dependent decarboxylation of pyruvate and the reductive acetylation of lipoyl groups on the E2 component. Related references: PMID:17474719, PMID:19081061, PMID:7782287
• Ubiquitously expressed across tissues, reflecting its housekeeping role in cellular energy metabolism.
• Localized to the mitochondrial matrix, where the PDH complex is active.
• Subject to regulatory phosphorylation and acetylation, modulating enzymatic activity in response to metabolic demands.
• Mutations in PDHA1 are associated with primary mitochondrial diseases, including Leigh syndrome, highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50–150 of human PDHA1 (P08559); cross-reactivity with mouse and rat PDHA1 (Swiss-Prot P35486 and P26284) is expected based on sequence alignment.
• For Western blot, the predicted molecular weight is 43 kDa; validate detection in mitochondrial-enriched fractions or whole-cell lysates from relevant cell lines/tissues.
• For immunofluorescence/immunocytochemistry, consider using a mitochondrial stain to confirm subcellular localization.
• For ELISA, titrate the antibody for optimal signal-to-noise ratio using recombinant PDHA1 protein or PDH complex as capture antigen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, from concept to clinic. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for metabolic biomarker research. For targets like PDHA1, central to pyruvate dehydrogenase complex-related disorders, CamelBio can assist with rare-target raw-material sourcing and custom antibody development to accelerate your diagnostic assay pipeline.