Anti-PDGFRB Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P09619

Core Product Specifications and Parameters

Biological Background: PDGFRB Function and Localization

• Encodes the beta-type platelet-derived growth factor receptor, a tyrosine-protein kinase that acts as cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB.
• Essential for embryonic development, regulating cell proliferation, survival, differentiation, chemotaxis, and migration.
• Critical for blood vessel development by promoting proliferation, migration, and recruitment of pericytes and smooth muscle cells to endothelial cells.
• Plays a role in vascular smooth muscle cell migration and neointima formation at vascular injury sites.
• Required for normal kidney glomerulus development, including pericyte (mesangial cell) recruitment and branched capillary network formation.
• Ligand binding activates multiple signaling cascades, including PLCG1, PI3K/AKT1, and RAS/MAPK pathways, leading to cytoskeletal rearrangement and membrane ruffle formation.
• Down-regulated by protein phosphatases and rapid internalization of the activated receptor.
• Alternative splicing produces multiple isoforms; implicated in various cancers and other diseases via chromosomal rearrangements and mutations.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody targets a region within amino acids 200–300 of human PDGFRB, located in the extracellular domain. This region is predicted to be conserved across species, consistent with the reported cross-reactivity with mouse and rat.
• For western blot analysis, expect a band around 124 kDa for the full-length glycosylated receptor. Post-translational modifications and proteolytic processing may produce additional bands; validation in the relevant cell or tissue lysate is recommended.
• For immunohistochemistry and immunofluorescence, consider antigen retrieval methods and appropriate controls to confirm specificity.
• Although not tested for other applications, this polyclonal antibody may be suitable for immunoprecipitation or other assays with proper optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic developers and research teams worldwide to provide reliable access to critical raw materials like high-quality antibodies for receptor tyrosine kinase targets. For projects focusing on angiogenesis, oncology, or cardiovascular biology, we offer validated antibody pairs, bulk polyclonal and monoclonal candidate antibodies, and ancillary reagents. Our sourcing expertise ensures you can rapidly secure the components needed to transition from concept to clinic.