Anti-PDGFRB Polyclonal Antibody for WB - P09619

Core Product Specifications and Parameters

Biological Background: PDGFRB Function and Localization

• PDGFRB encodes the platelet-derived growth factor receptor beta, a receptor tyrosine kinase that binds homodimeric PDGFB and PDGFD and heterodimers formed by PDGFA and PDGFB.
• It plays essential roles in embryonic development, cell proliferation, survival, differentiation, chemotaxis, and migration.
• PDGFRB is critical for blood vessel development, promoting proliferation, migration, and recruitment of pericytes and smooth muscle cells to endothelial cells; it also drives neointima formation at vascular injury sites.
• Required for normal kidney glomerulus development, it facilitates the formation of a branched capillary network and recruitment of mesangial cells.
• Ligand binding activates multiple signaling cascades, including PLCG1-mediated calcium mobilization, PI3K-AKT, and RAS-MAPK pathways, leading to cytoskeletal reorganization and membrane ruffling.
• The receptor is located at the cell membrane, cytoplasmic vesicles, and lysosome lumen; signaling is downregulated by dephosphorylation and rapid internalization.
• Post-translational modifications include disulfide bond formation, glycosylation, phosphorylation, and ubiquitination (Keywords: Glycoprotein, Phosphoprotein, Ubl conjugation). Alternative splicing has been reported.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 900–1000) of human PDGFRB, which may detect full-length and potential cleaved or truncated forms.
• For Western blot (WB), the predicted molecular weight is 124 kDa; consider using sufficient protein loading and optimized transfer conditions due to the high molecular weight.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate the antibody in tissues or cells with known PDGFRB expression, such as vascular smooth muscle or kidney glomeruli.
• For ELISA, optimize coating antigen concentrations and include appropriate controls to ensure specificity.
• Cross-reactivity with mouse and rat suggests suitability for rodent model studies; however, verify sequence homology of the immunogen region in these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-PDGFRB polyclonal antibody is ideal for oncology, cardiovascular, and renal disease research, serving as a robust component in assay development. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your IVD projects.