Anti-PDGFRA/PDGFR alpha Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P16234

Core Product Specifications and Parameters

Biological Background: PDGFR alpha Function and Localization

• PDGFR alpha (CD140a, PDGFR2) is a cell-surface tyrosine-kinase receptor encoded by the PDGFRA gene, serving as the high-affinity receptor for platelet-derived growth factors PDGFA, PDGFB, and PDGFC.
• Ligand binding induces receptor dimerization and autophosphorylation, activating key downstream signaling cascades including RAS/MAPK, PI3K/AKT, PLCG1, and STAT pathways to regulate cell proliferation, survival, migration, and chemotaxis.
• Plays an essential role in embryonic development, particularly in skeletal development, cephalic closure, and gastrointestinal tract morphogenesis, and is also required for mesenchymal stem cell differentiation.
• Exhibits context-dependent dual roles, capable of promoting or inhibiting cell proliferation and migration depending on the cellular environment and ligand composition.
• Involved in wound healing and platelet biology, contributing to platelet activation, agonist secretion, and thrombin-induced aggregation.
• Alternatively spliced isoforms exist; the canonical full-length receptor localizes predominantly to the cell membrane, but is also found in the Golgi apparatus and primary cilium, indicating intracellular trafficking and signaling compartmentalization.
• Widely expressed across tissues, with detection in brain, fibroblasts, smooth muscle, heart, platelets, and both normal and tumor colon tissues, highlighting its roles in both normal physiology and oncogenesis.
• Post-translationally modified by phosphorylation, glycosylation, disulfide bond formation, and ubiquitination, which regulate receptor activity, stability, and signaling output.

Experimental Guidance and Technical Tips

• The immunogen covers the intracellular domain (aa 896-1059), making this antibody suitable for detecting total PDGFR alpha in Western blotting under reducing conditions; the predicted band is ~123 kDa, but glycosylation may cause a shift.
• For immunohistochemistry on paraffin sections (IHC-P), consider heat-induced antigen retrieval as membrane receptors can be masked; test multiple retrieval buffers to optimize signal.
• When used in immunofluorescence/ICC, expect membranous and possibly perinuclear (Golgi) staining; validate specificity with appropriate controls, such as PDGFRA knockdown or knockout cell lines.
• This antibody may recognize both human and rodent (mouse/rat) PDGFR alpha, but cross-reactivity with other species should be verified experimentally.
• For ELISA, performance depends on the coating method and antibody pairing; pilot testing with recombinant PDGFR alpha protein is recommended to establish optimal working concentrations.

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