Anti-PDGFRα/CD140a Rabbit Monoclonal Antibody () for Flow Cytometry - P16234

Core Product Specifications and Parameters

Biological Background: PDGFRα Function and Localization

• PDGFRα (CD140a, PDGFR2) is a receptor tyrosine kinase that binds platelet-derived growth factors (PDGFA, PDGFB, PDGFC) and regulates embryonic development, cell proliferation, survival, and chemotaxis.
• It is essential for normal skeleton development, cephalic closure, and formation of the gastrointestinal mucosa, as well as differentiation of bone marrow-derived mesenchymal stem cells.
• Ligand binding triggers activation of key signaling cascades, including PI3K-AKT, RAS-MAPK, and STAT pathways, influencing gene expression and cellular responses.
• PDGFRα also participates in platelet activation, secretion of granule agonists, and thrombin-induced platelet aggregation.
• The receptor localizes to the cell membrane, primary cilia, and Golgi apparatus, integrating extracellular signals with intracellular trafficking.
• Widely expressed in brain, fibroblasts, smooth muscle, heart, and platelets; detected in primary and metastatic colon tumors and normal colon tissue.
• Post-translational modifications include glycosylation, phosphorylation, and ubiquitination; alternative splicing yields isoforms with molecular weights of approximately 24, 82, and 123 kDa.
• Annotated as a proto-oncogene and developmental protein, PDGFRα is involved in host-virus interactions and is a target for tyrosine kinase inhibitors in various cancers.

Experimental Guidance and Technical Tips

• This antibody is provided as a ready-to-use conjugate with for direct flow cytometry; perform titration experiments to determine the optimal dilution for your specific cell model.
• For surface staining of live cells, no permeabilization is required; include appropriate isotype controls and fluorescence-minus-one (FMO) controls.
• Given cross-reactivity with mouse, this antibody can be used in both human and mouse systems; validate performance in the relevant species and tissue context.
• The immunogen spans the extracellular domain (aa 24–524); consider that soluble receptor isoforms or ligand competition may affect staining intensity.
• For sensitive detection, optimize cell number, staining volume, and incubation time, and use freshly harvested cells to minimize receptor internalization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents—connecting diagnostic manufacturers and labs with reliable tools for receptor tyrosine kinase research. Whether you need this anti-PDGFRα clone, custom conjugations, or rare target raw materials, our one-stop platform supports your journey from concept to clinic with dedicated technical consulting and supply-chain efficiency.