Anti-PDGFD Rabbit Polyclonal Antibody for WB, ELISA - Q9GZP0

Core Product Specifications and Parameters

Biological Background: PDGFD Function and Localization

• Platelet-derived growth factor D (PDGFD), also known as iris-expressed growth factor (IEGF) or spinal cord-derived growth factor B (SCDGF-B), is a secreted growth factor belonging to the PDGF family.
• PDGFD plays an essential role in embryonic development by regulating cell proliferation, migration, survival, and chemotaxis; it acts as a potent mitogen for cells of mesenchymal origin.
• The protein is crucial for wound healing, where it induces macrophage recruitment, increases interstitial pressure, and promotes blood vessel maturation during angiogenesis.
• PDGFD is predominantly secreted and functions in an autocrine or paracrine manner after proteolytic activation by cleavage on pair of basic residues.
• Tissue expression profiling reveals high levels in the heart, pancreas, adrenal gland, and ovary; lower expression is detected in placenta, liver, kidney, prostate, testis, small intestine, spleen, and colon.
• In the kidney, PDGFD is expressed by glomerular visceral epithelial cells and medial smooth muscle cells of arteries; its aberrant expression is associated with chronic obstructive nephropathy and mesangial proliferative glomerulonephritis.
• The protein is a disulfide-linked homodimer and undergoes alternative splicing; it is also classified as a proto-oncogene due to its involvement in cell proliferation and cancer.
• Increased and more diffuse expression is observed in prostate, ovarian, lung, renal, and brain cancer-derived cell lines, suggesting a role in tumor biology.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 250–350 of human PDGFD (NP_079484.1); consider epitope accessibility in the native protein context, especially under reducing versus non-reducing conditions due to disulfide bonds.
• For Western blot (WB), validate the antibody in relevant sample systems expressing PDGFD (e.g., heart, pancreas, or cancer cell lines) and be aware that the observed band may shift depending on glycosylation and proteolytic processing; the predicted molecular weight of the full-length precursor is approximately 43 kDa.
• In ELISA applications, the polyclonal nature may enhance detection sensitivity, but it is advised to test cross-reactivity with closely related PDGF family members if specificity is critical.
• If investigating tissue or cell-type-specific expression, include appropriate controls (e.g., tissues with known high or low expression) and consider that PDGFD is secreted, so intracellular detection may require optimal permeabilization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies high-quality IVD raw materials, including validated antibody pairs and optimized polyclonal and monoclonal antibodies, supporting diagnostic developers and research labs. This PDGFD Rabbit pAb is part of our growth factor and oncology-related portfolio, ideal for studies on development, wound healing, and cancer biology. Beyond antibodies, we offer bulk ancillary reagents and expedited sourcing of rare target raw materials, serving as your reliable partner from concept to clinic.