Anti-PDGFD Rabbit Polyclonal Antibody for WB and ELISA - Q9GZP0

Core Product Specifications and Parameters

Biological Background: PDGFD Function and Localization

• Platelet-derived growth factor D (PDGFD), also designated iris-expressed growth factor (IEGF) and spinal cord-derived growth factor B (SCDGF-B), is a secreted glycoprotein encoded by the PDGFD gene.
• PDGFD functions as a potent mitogen and chemoattractant for cells of mesenchymal origin, playing critical roles in cell proliferation, migration, survival, and embryonic development.
• It contributes significantly to wound healing by inducing macrophage recruitment, elevating interstitial pressure, and promoting blood vessel maturation during angiogenesis.
• PDGFD has been implicated in pathological conditions, including mesangial proliferative glomerulonephritis, characterized by monocyte/macrophage influx and extracellular matrix accumulation.
• Subcellular localization is primarily extracellular; the protein is secreted from producing cells.
• Tissue expression is highest in the heart, pancreas, adrenal gland, and ovary, with lower levels in placenta, liver, kidney, prostate, testis, small intestine, spleen, and colon. In the kidney, it is expressed by visceral epithelial cells of glomeruli.
• Enhanced and widespread expression is observed in many cancer-derived cell lines and prostate carcinoma, supporting a role in tumorigenesis.
• UniProt lists molecular weight at 42848 Da; the product recognizes a band around 43 kDa due to post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 19-307 of human PDGFD (NP_079484.1). Researchers should verify that this region is present in their target form or species variant.
• For Western blot, the predicted molecular weight is approximately 43 kDa. Use reducing conditions and consider that the protein is secreted; collect cell culture supernatants or tissue lysates appropriately.
• Cross-reactivity with mouse has been observed; for other species, sequence homology should be checked and experimental validation performed.
• ELISA applications may require optimization of coating antigen and detection conditions; consider using recombinant PDGFD as a standard.
• Given its role as a secreted growth factor, confirm expression in your experimental system; serum-containing media may contain PDGFD from supplements.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and research institutions, offering a wide range of high-quality raw materials including this PDGFD rabbit polyclonal antibody. Our portfolio extends to validated antibody pairs, bulk quantities of monoclonal and polyclonal antibodies, and ancillary reagents to support your developmental biology, wound healing, and oncology projects. Whatever your target—whether routine or rare—CamelBio bridges the gap from concept to clinic with reliable procurement and expert technical support.