Anti-PDGFB Rabbit Monoclonal Antibody for WB, IHC-P, IF-P, ELISA - P01127

Core Product Specifications and Parameters

Biological Background: PDGFB Function and Localization

• PDGFB encodes platelet-derived growth factor subunit B, also known as PDGF-2, PDGF B chain, and proto-oncogene c-Sis; the mature protein is a secreted growth factor of ~27 kDa (SWISS: P01127).
• PDGFB functions as a potent mitogen for mesenchymal cells, playing essential roles in embryonic development, cell proliferation, migration, survival, and chemotaxis (PubMed:26599395). It is critical for pericyte and vascular smooth muscle cell recruitment in multiple organs. Related references: PMID:26599395
• PDGFB can form homodimers (PDGF-BB) or heterodimers with PDGFA (PDGF-AB), modulating its signaling activities; it is a member of the PDGF/VEGF growth factor family.
• Subcellular localization is primarily secreted, consistent with its role as an extracellular ligand that binds to PDGF receptors (PDGFRα/β) on target cells.
• Post-translational modifications include disulfide-linked dimerization and glycosylation, which are essential for its biological activity and stability.
• Tissue expression is highest in heart, brain (substantia nigra), placenta, and fetal kidney, with moderate levels in hippocampus, skeletal muscle, kidney, and lung, reflecting its broad developmental and physiological roles.
• PDGFB is implicated in wound healing, angiogenesis, and various pathologies including cancer (as a proto-oncogene). Chromosomal rearrangements involving PDGFB are associated with certain malignancies.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic peptide spanning amino acids 142-241, a region that may be conserved across human, mouse, and rat PDGFB, supporting the observed cross-reactivity.
• For Western blotting (WB), use reducing and non-reducing conditions to distinguish monomeric (~27 kDa) and dimeric (PDGF-BB) forms. Validate in relevant cell or tissue lysates; consider using placenta or fetal kidney as positive controls.
• In immunohistochemistry (IHC-P), antigen retrieval and antibody dilution may require optimization. Tissues with high PDGFB expression (e.g., placenta) can serve as positive controls.
• For immunofluorescence (IF-P), confirm specificity with appropriate negative controls and consider co-staining with PDGFR markers to demonstrate ligand-receptor interactions.
• For ELISA, ensure the antibody pair is validated; this monoclonal may be used as a capture or detection antibody depending on the assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material solutions, helping diagnostic developers and research labs accelerate projects from concept to clinic. Our portfolio includes validated monoclonal antibodies like this anti-PDGFB antibody, along with complementary reagents such as recombinant proteins, ELISA kits, and ancillary buffers. Whether you need bulk quantities, customized conjugations, or technical consultation, CamelBio is your trusted partner in growth factor, oncology, and cardiovascular research.