Anti-PDGFA Polyclonal Antibody for IHC-P, ELISA - P04085

Core Product Specifications and Parameters

Biological Background: PDGFA Function and Localization

• Platelet-derived growth factor subunit A (PDGFA, also known as PDGF-1 or PDGF-A chain) is a secreted growth factor that belongs to the PDGF family.
• PDGFA acts as a potent mitogen for mesenchymal cells and plays essential roles in embryonic development, including lung alveolar septum formation, gastrointestinal tract development, and spermatogenesis.
• It is required for normal oligodendrocyte development and myelination in the spinal cord and cerebellum.
• PDGFA contributes to wound healing by promoting cell proliferation, migration, survival, and chemotaxis.
• Signaling can be modulated through heterodimerization with PDGFB (by similarity).
• The protein is secreted and undergoes cleavage on a pair of basic residues to generate the active form.
• Post-translational modifications include disulfide bond formation and glycosylation; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 21–110 of human PDGFA, a region likely to be accessible in the mature protein, making this antibody suitable for detecting secreted and processed forms.
• For IHC-P applications, consider optimizing antigen retrieval conditions and validating staining patterns in relevant human or mouse tissues known to express PDGFA (e.g., lung, spinal cord, testes).
• In ELISA setups, include appropriate controls to confirm specificity, especially if testing samples containing heterodimeric PDGF complexes.
• Since the antibody is polyclonal, lot-to-lot consistency should be verified when transitioning to new batches.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with reliable access to IVD raw materials for growth factor targets like PDGFA. We offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to support assay development from concept to clinic. For niche or hard-to-source targets, our team can facilitate custom procurement to meet your program's needs.