Anti-PDE8A Rabbit Polyclonal Antibody for WB, ELISA - O60658

Core Product Specifications and Parameters

Biological Background: PDE8A Function and Localization

• PDE8A is a high-affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase, also known as HsT19550, with multiple isoforms generated by alternative splicing (UniProt keywords: Alternative splicing).
• The enzyme hydrolyzes the second messenger cAMP, playing a key role in regulating numerous physiological processes and maintaining basal cAMP levels; it is particularly implicated in cAMP regulation during germ cell development (PubMed:18983167). Related references: PMID:18983167
• PDE8A binds to RAF1 and reduces its inhibitory phosphorylation at Ser-259, thereby stimulating RAF1-dependent EGF-activated ERK signaling (PubMed:23509299). Related references: PMID:23509299
• This phosphodiesterase protects against hydrogen peroxide- and staurosporine-induced cell death, highlighting a role in cellular survival pathways (PubMed:23509299). Related references: PMID:23509299
• Tissue expression analysis shows PDE8A is widely distributed, with highest levels in testis, ovary, small intestine, and colon; it is absent from thymus and peripheral blood leukocytes.
• UniProt keywords annotate it as a metal-binding phosphoprotein with a known 3D structure, supporting its functional characterization.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1–120 at the N-terminus of human PDE8A; thus, the antibody is expected to recognize epitopes within this region and may detect all isoforms containing this sequence.
• For Western blotting (WB), consider using a dilution series to determine the optimal working concentration; the predicted molecular weight is ~93 kDa, but post-translational modifications or isoform variations may shift the band.
• For ELISA applications, validate the antibody against recombinant or native protein in the relevant sample matrix; cross-reactivity with mouse and rat has been noted, so consider these species for multispecies studies.
• As with any polyclonal antibody, lot-to-lot consistency should be independently verified, and appropriate negative controls are recommended for specific signal confirmation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic developers and researchers streamlined access to a comprehensive catalog of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents. For targets like PDE8A involved in cAMP signaling and cell survival, we supply bulk quantities and can source rare reagents to accelerate your assay development from concept to clinic.