Anti-PDE7A Polyclonal Antibody for WB, ELISA - Q13946

Core Product Specifications and Parameters

Biological Background: PDE7A Function and Localization

• PDE7A is a high-affinity cAMP-specific phosphodiesterase that hydrolyzes the second messenger cAMP, a key regulator of numerous physiological processes. Related references: PMID:19350606, PMID:8389765, PMID:9195912
• It belongs to the cyclic nucleotide phosphodiesterase family, specifically the PDE7 subfamily, and is encoded by the PDE7A gene (GeneID: 5150) with alternative splicing producing multiple isoforms.
• The protein is primarily localized in the cytoplasm and cytosol, consistent with its role in regulating intracellular cAMP levels.
• PDE7A is abundantly expressed in skeletal muscle, with lower levels detected in the heart and brain, and is also expressed in T-cell lines, suggesting a role in muscle and immune cell signal transduction. Related references: PMID:9195912
• It is a metal-binding phosphoprotein, and its activity may be modulated by phosphorylation, as indicated by proteomics identification of phosphorylation sites.
• The protein has a predicted molecular weight of approximately 55.5 kDa (UniProt: 55505 Da), though the immunogen-based antibody detects a band at 56 kDa by western blot, reflecting the endogenous protein weight.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fragment spanning amino acids 345-456 of human PDE7A; consider this region’s conservation when assessing cross-reactivity with mouse and rat orthologs.
• For western blot (WB), use lysates from skeletal muscle or heart tissue where PDE7A is abundantly expressed; validate in your specific sample system to confirm specificity.
• For ELISA applications, this polyclonal antibody may be used as a capture or detection reagent; optimize coating concentrations and blocking conditions experimentally.
• The predicted molecular weight is ~56 kDa; include appropriate molecular weight markers and consider the possibility of post-translational modifications affecting migration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials and technical support, serving as a one-stop partner for diagnostic manufacturers and research laboratories. This anti-PDE7A polyclonal antibody supports investigations into cAMP signaling, muscle biology, and immune cell regulation, with applications in Western blotting and ELISA. Through validated antibody pairs, optimized monoclonal and polyclonal antibodies, and access to rare-target raw materials, CamelBio accelerates your development programs from concept to clinical application.