Anti-PDE6D Polyclonal Antibody for WB, IF/ICC, ELISA - O43924

Core Product Specifications and Parameters

Biological Background: PDE6D Function and Localization

• PDE6D, also known as Protein p17, is the delta subunit of retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase, encoded by the PDE6D (PDED) gene (UniProt O43924).
• Promotes the release of prenylated target proteins from cellular membranes, facilitating their trafficking and subcellular redistribution.
  Related references: PMID:9712853
• Modulates the activity of prenylated or palmitoylated Ras family members by regulating their subcellular location, impacting signaling pathways (PubMed:22002721, 23698361).
  Related references: PMID:22002721, PMID:23698361
• Required for normal ciliary targeting of farnesylated proteins, such as INPP5E, linking PDE6D to ciliopathies like Joubert syndrome (PubMed:24166846).
  Related references: PMID:24166846
• Functions as a GTP-specific dissociation inhibitor (GDI) for certain small GTPases, modulating their membrane association and activity (By similarity).
• Enhances ARL3 GTP affinity and stabilizes its GTP-bound state, implicating PDE6D in ARL3-mediated ciliary trafficking (By similarity).
• Subcellularly localized to the cytosol, cytoplasmic vesicle membranes, cytoskeleton, and cilium basal body, consistent with roles in membrane trafficking and ciliary function.
• Widely expressed in tissues including spleen, prostate, testis, ovary, small intestine, colon, retina, and peripheral blood, with critical roles in visual sensory transduction.

Experimental Guidance and Technical Tips

• The immunogen (AA 1-150) covers the N-terminal region of PDE6D; verify detection of full-length and any isoforms in relevant sample systems.
• For Western blot (WB), consider using positive lysates from human, mouse, or rat tissues with high PDE6D expression (e.g., retina, testis) and validate band specificity at ~17 kDa.
• In immunofluorescence/immunocytochemistry (IF/ICC), expect staining in the cytoplasm, and possibly at the ciliary basal body; include appropriate fixation and permeabilization protocols.
• For ELISA, use recombinant PDE6D protein as a positive control to assess antibody reactivity; cross-reactivity with mouse and rat orthologs should be confirmed.
• Because PDE6D is involved in prenylation-dependent trafficking, consider co-localization studies with prenylation markers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a one-stop access to IVD raw materials. This PDE6D antibody supports research in vision biology, ciliopathies, and RAS signaling, and we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing tailored to your assay development needs.