Anti-PDE6B Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P35913

Core Product Specifications and Parameters

Biological Background: PDE6B Function and Localization

• PDE6B, also known as rd1, PDEB, RP40, CSNB3, CSNBAD2, and GMP-PDEbeta, is the catalytic beta subunit of rod-specific cGMP phosphodiesterase (PDE6). Related references: PMID:20940301
• The PDE6 holoenzyme comprises PDE6A and PDE6B catalytic subunits and two inhibitory PDE6G gamma subunits. Upon light activation, GNAT1 relieves gamma subunit inhibition, enabling PDE6B to hydrolyze cGMP, a critical step in visual signal transduction. Related references: PMID:8394174
• Decreased cytosolic cGMP levels lead to closure of cGMP-gated cation channels and rod hyperpolarization, converting light signals into electrical responses.
• PDE6B localizes to the photoreceptor outer segment membrane, where phototransduction occurs.
• The protein is subject to multiple post-translational modifications, including prenylation, acetylation, and alternative splicing, which may regulate its function and localization.
• PDE6B contains metal-binding sites for magnesium, manganese, and zinc, essential for its catalytic activity.
• It is involved in retinal circadian rhythm photoentrainment by modulating light-induced phase-shifts of the retina clock.
• Mutations in PDE6B cause congenital stationary night blindness (CSNB) and retinitis pigmentosa (RP), highlighting its importance in retinal health.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-190) of human PDE6B, which may yield reactivity with both full-length and truncated isoforms.
• For Western blot (WB), the predicted molecular weight is ~98 kDa; consider using a reducing gel and appropriate transfer conditions for large proteins.
• For immunohistochemistry (IHC-P), validate antigen retrieval and antibody dilution in appropriate positive control tissues such as retina.
• Cross-reactivity is reported for human, mouse, and rat; perform species-specific validation for other species.
• As a polyclonal antibody, lot-to-lot variability may occur; consider including positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials like this anti-PDE6B antibody, supporting vision research and retinal disease studies. Our portfolio includes validated antibody pairs, optimized polyclonal antibodies, and bulk reagents, simplifying your supply chain from discovery to clinical application.