Anti-PDE6A Rabbit Polyclonal Antibody for WB, ELISA - P16499

Core Product Specifications and Parameters

Biological Background: PDE6A Function and Localization

• PDE6A encodes the rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha, also known as PDE V-B1, a critical enzyme in the visual phototransduction cascade.
• The protein serves as a catalytic subunit in the rod-specific PDE6 complex, which hydrolyzes cGMP to 5'-GMP. The holoenzyme comprises two catalytic subunits (PDE6A and PDE6B) and two inhibitory gamma subunits (PDE6G). Related references: PMID:20940301
• Light-activated transducin (GNAT1) relieves PDE6G-mediated inhibition, activating PDE6A/PDE6B to reduce cytosolic cGMP. This closure of cGMP-gated cation channels hyperpolarizes rod photoreceptors, mediating visual signal transduction.
• PDE6A is anchored to the photoreceptor outer segment membrane via prenylation and methylation modifications, facilitating its precise localization for rapid signal amplification.
• Keywords associated with PDE6A include hydrolase, lipoprotein, magnesium, metal-binding, zinc, sensory transduction, vision, and retinitis pigmentosa, underscoring its role in retinal function and pathology.
• Mutations in PDE6A are linked to retinitis pigmentosa, an inherited retinal degenerative disease, highlighting its essential role in photoreceptor survival.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 656-860 of human PDE6A. When performing WB, a band around 100 kDa may be observed; consider using reducing conditions and appropriate molecular weight markers.
• For WB in mouse and rat samples, validate cross-reactivity since the amino acid sequence similarity is high but not identical; consider running a human positive control.
• In ELISA applications, coating antigen (e.g., recombinant fragment or full-length protein) in a direct or sandwich format may be used; optimize coating buffer and blocking conditions.
• As a polyclonal antibody, it may recognize multiple epitopes; pre-adsorption may be considered if non-specific binding is observed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-PDE6A polyclonal antibody supports studies in retinal disorders, phototransduction signaling, and retinitis pigmentosa, meeting the detection needs of assay developers. For tailored sourcing solutions from concept to clinic, contact our team for personalized support.