Anti-PDE5A Rabbit Polyclonal Antibody for WB, ELISA - O76074

Core Product Specifications and Parameters

Biological Background: PDE5A Function and Localization

• PDE5A is a cGMP-specific 3',5'-cyclic phosphodiesterase that catalyzes the hydrolysis of cGMP to 5'-GMP, thereby modulating intracellular cGMP levels (PubMed:15489334, PubMed:9714779). Related references: PMID:15489334 PMID:9714779
• Specifically, PDE5A regulates nitric oxide (NO)-generated cGMP, playing a critical role in smooth muscle relaxation and cardiovascular signaling (PubMed:15489334). Related references: PMID:15489334
• It is highly expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle, and pancreas, with lower levels in brain, liver, and lung.
• The enzyme is a homodimer with each subunit containing a catalytic domain, a cGMP-binding regulatory domain, and a phosphorylation site for protein kinase G, allowing allosteric regulation by cGMP.
• PDE5A binds zinc and cGMP, and its activity is inhibited by drugs such as sildenafil, making it a therapeutic target for erectile dysfunction and pulmonary hypertension.
• Alternative splicing of PDE5A results in multiple isoforms that differ in their N-terminal regions, affecting tissue distribution and regulatory properties.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-150), so the antibody may detect full-length and some isoforms; verify in the relevant sample system.
• For Western blot, the predicted molecular weight is ~100 kDa; consider using appropriate gel percentage and loading controls.
• Validate cross-reactivity in mouse and rat tissues as the antibody is reported to react with these species.
• For ELISA, optimize coating concentration and sample dilutions; the antibody can be paired with a detection antibody for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive sourcing solutions for IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For targets like PDE5A, which are integral to cGMP signaling and cardiovascular research, CamelBio bridges the gap from discovery to diagnostic development with reliable supply chains and technical support.