Anti-PDE4DIP Polyclonal Antibody for WB, ELISA - Q5VU43

Core Product Specifications and Parameters

Biological Background: Myomegalin Function and Localization

• Myomegalin (PDE4DIP/CMYA2) is a large coiled-coil protein encoded by the PDE4DIP gene, which undergoes alternative splicing and is implicated in chromosomal rearrangements.
• Serves as an anchor protein that sequesters components of the cAMP-dependent signaling pathway at the Golgi apparatus and/or centrosomes (By similarity).
• Promotes microtubule assembly and participates in microtubule dynamics, acting at the Golgi apparatus or centrosome depending on the cellular context.
  Related references:
  PMID:25217626 PMID:27666745 PMID:28814570
• In complex with AKAP9, recruits CAMSAP2 to the Golgi apparatus, tethering non-centrosomal minus-end microtubules to the Golgi — a mechanism critical for polarized cell migration.
  Related references:
  PMID:27666745 PMID:28814570
• Together with AKAP9, EB1/MAPRE1, and CDK5RAP2, facilitates microtubule nucleation and extension from the centrosome, impacting directed cell migration, mitotic spindle orientation, and cell-cycle progression.
  Related references:
  PMID:29162697
• Predominantly localized to the Golgi apparatus, centrosome, and cytoplasmic microtubule network, with high expression in adult and fetal heart, skeletal muscle, and lower levels in brain and placenta.
• Post-translationally regulated by phosphorylation (Phosphoprotein keyword) and features multiple coiled-coil domains that mediate protein–protein interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–310), which may be conserved in mouse; confirm cross-reactivity in your experimental system.
• The full-length protein has a high molecular weight (~265 kDa); for WB, consider using low-percentage SDS-PAGE gels (e.g., 6–8%), extended transfer times, and appropriate prestained markers.
• Alternative splicing generates multiple isoforms; validate the expected band pattern using known positive controls (e.g., heart or skeletal muscle lysates).
• For ELISA, optimize coating buffers and blocking conditions, as the polyclonal nature may require titration to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw-material sourcing, supporting diagnostic manufacturers and research labs with validated antibodies, bulk ancillary reagents, and rare target discovery. From optimized polyclonal antibodies like this anti-PDE4DIP reagent to custom monoclonal development, we bridge supply gaps for cardiac and muscle biology research. Contact us to explore how our procurement capabilities can streamline your assay development pipeline.