Anti-PDE4C Polyclonal Antibody for IF/ICC, ELISA - Q08493

Core Product Specifications and Parameters

Biological Background: PDE4C Function and Localization

• PDE4C encodes a cAMP-specific phosphodiesterase that hydrolyzes the second messenger cAMP, a crucial regulator of many physiological processes.
• Belongs to the cyclic nucleotide phosphodiesterase family; multiple isoforms are produced by alternative splicing.
• Localizes to cell projections, specifically the cilium, pointing to a role in compartmentalized cAMP signaling within these organelles.
• Shows tissue-specific expression: present in various tissues but absent from immune system cells, suggesting tailored regulation of cAMP levels.
• Its catalytic activity depends on divalent metal ions such as manganese and zinc.
• Identified as a phosphoprotein in proteomics studies, indicating regulation by phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 638–712), which may allow detection of multiple isoforms if the sequence is conserved.
• For IF/ICC, optimize fixation and permeabilization protocols, and validate in ciliated cell models given PDE4C's cilium localization.
• In ELISA, assess performance as capture or detection antibody using recombinant PDE4C or relevant cell lysates; confirm specificity with appropriate controls.
• Being a polyclonal antibody, monitor lot-to-lot consistency and consider re-validation when switching lots.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to IVD raw materials, including this high-quality anti-PDE4C polyclonal antibody for cAMP signaling research. Beyond validated antibodies, we support your projects with optimized antibody pairs, bulk ancillary reagents, and sourcing of rare-target raw materials, offering a comprehensive supply solution to accelerate your assay development.