Anti-PDE4B Polyclonal Antibody for WB, ELISA - Q07343

Core Product Specifications and Parameters

Biological Background: PDE4B Function and Localization

• PDE4B (3',5'-cyclic-AMP phosphodiesterase 4B), also known as DPDE4 or PDE32, is a member of the PDE4 family that specifically hydrolyzes cAMP, a key second messenger. It plays a role in mediating the central nervous system effects of therapeutic agents, including antidepressants, antiasthmatics, and anti-inflammatory drugs. Related references: PMID:15260978
• The enzyme contains a conserved catalytic domain that binds divalent metal ions, likely zinc, which is essential for its phosphodiesterase activity.
• PDE4B undergoes alternative splicing, producing multiple isoforms, including a brain-specific variant highly expressed in the central nervous system.
• It is localized to both the cytoplasm and the cell membrane, allowing it to regulate compartmentalized cAMP signaling near membrane-associated complexes.
• PDE4B is expressed in a variety of tissues, including brain, heart, lung, skeletal muscle, and white blood cells, highlighting its broad physiological roles.
• The protein is regulated by phosphorylation, which modulates its activity and subcellular localization, contributing to signal integration.
• Given its expression in white blood cells and involvement in anti-inflammatory drug action, PDE4B is a significant pharmacological target for respiratory and inflammatory diseases.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 636-736) of human PDE4B (NP_002591.2), which is conserved across human, mouse, and rat, likely accounting for the observed cross-reactivity.
• For Western blotting, consider using whole cell lysates from tissues with high PDE4B expression (e.g., brain or lung) to confirm a specific band at ~83 kDa; inclusion of a positive control is recommended.
• In ELISA, validate the antibody with recombinant PDE4B protein or the immunogen peptide to determine optimal coating concentrations and antibody dilutions.
• As a polyclonal antibody, it may recognize multiple isoforms; confirm isoform specificity using isoform-specific controls or by comparing band patterns across cell types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers by offering streamlined, one-stop access to IVD raw materials like this anti-PDE4B antibody. We supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target sourcing to accelerate your cAMP signaling or anti-inflammatory drug research. Let our supply chain expertise bridge your development needs, from concept to clinical application.