Anti-PDE3B Rabbit Polyclonal Antibody for WB, ELISA - Q13370

Core Product Specifications and Parameters

Biological Background: PDE3B Function and Localization

• PDE3B, also known as CGIP1 or CGI-PDE B, is a dual-specificity cyclic nucleotide phosphodiesterase that hydrolyzes both cAMP and cGMP, thereby playing a central role in modulating intracellular signaling pathways (PubMed:14592490, PubMed:21393242). Related references: PMID:14592490 PMID:21393242
• It regulates angiogenesis by inhibiting the cAMP-dependent guanine nucleotide exchange factor RAPGEF3 and downstream PI3Kγ-mediated signaling (PubMed:21393242). Related references: PMID:21393242
• PDE3B controls cardiac contractility by reducing cAMP concentration in cardiomyocytes, as inferred from homologous studies.
• The protein localizes to cellular membranes and contains transmembrane helices, consistent with its membrane association.
• Predominantly expressed in adipose tissues, PDE3B is also subject to alternative splicing and phosphorylation, suggesting tissue-specific and post-translational regulation.
• Its activity relies on metal binding (e.g., magnesium), and it has been identified in large-scale proteomic analyses.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 400–660 of human PDE3B, a conserved region that likely confers cross-reactivity with mouse samples as specified.
• For Western blot, expect a band at approximately 124 kDa; consider using 8% SDS-PAGE and optimized transfer conditions for high molecular weight proteins.
• In ELISA applications, titration of coating antigen and antibody dilution is recommended to achieve optimal signal-to-noise ratios.
• Given the membrane localization of PDE3B, sample preparation involving membrane protein extraction may improve detection sensitivity.

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CamelBio serves as your integrated sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies like this anti-PDE3B reagent, and bulk ancillary reagents to support diagnostic development in cardiovascular and metabolic research areas. Our one-stop platform simplifies procurement from concept to clinic, including rare target sourcing and technical consultation.