Anti-PDE3A Rabbit Polyclonal Antibody for WB, IP, ELISA - Q9Z0X4

Core Product Specifications and Parameters

Biological Background: PDE3A Function and Localization

• PDE3A (cGMP-inhibited 3',5'-cyclic phosphodiesterase A, CGI-PDE A) is a dual-substrate phosphodiesterase that hydrolyzes both cAMP and cGMP, key second messengers in cellular signaling. Related references: PMID:11420239
• Beyond its catalytic activity, PDE3A participates in an E2/17beta-estradiol-induced pro-apoptotic pathway by stabilizing the PDE3A/SLFN12 complex, leading to SLFN12 dephosphorylation and activation of its rRNA ribonuclease activity.
• The protein is localized to membranes and the cytosol, consistent with its transmembrane domains and roles in compartmentalized cyclic nucleotide signaling.
• PDE3A is a phosphoprotein subject to post-translational modifications, including phosphorylation and ubiquitination, which may regulate its activity and stability.
• It binds metal ions such as magnesium and manganese as cofactors for its phosphodiesterase activity.
• The gene encoding PDE3A is conserved across species, with the mouse protein sharing high homology with human PDE3A (GeneID: 5139).

Experimental Guidance and Technical Tips

• For Western blot, a band around 125 kDa is expected in mouse tissue lysates. Validate the antibody with appropriate positive and negative controls.
• In immunoprecipitation (IP), pre-clearing the lysate and using appropriate negative controls can help reduce non-specific binding.
• For ELISA, titration of both the capture/detection antibody and antigen is recommended to optimize signal-to-noise ratio.
• As a polyclonal antibody, slight batch-to-batch variation is normal; validation in your specific sample system is advised.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we provide diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-PDE3A rabbit polyclonal antibody supports studies on cyclic nucleotide signaling and apoptosis. Beyond this product, CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare-target raw materials to help accelerate your assay development and research.