Anti-PDE3A Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q14432

Core Product Specifications and Parameters

Biological Background: PDE3A Function and Localization

• PDE3A, also known as cGMP-inhibited phosphodiesterase A (CGI-PDE A), is a cyclic nucleotide phosphodiesterase that specifically hydrolyzes the second messengers cAMP and cGMP, playing a crucial role in regulating physiological processes such as cardiovascular function and insulin secretion.
  Related references:
  PMID:1315035 PMID:25961942 PMID:8155697

• Additionally, PDE3A exhibits catalytic activity toward cUMP, further broadening its substrate specificity.
  Related references:
  PMID:27975297

• Beyond its enzymatic role, PDE3A participates in an estradiol (E2)-induced pro-apoptotic signaling pathway independent of its phosphodiesterase activity. E2 stabilizes the PDE3A/SLFN12 complex in the cytosol, leading to SLFN12 dephosphorylation and activation of its ribonuclease activity, which ultimately triggers apoptosis. This pathway is implicated in placenta remodeling.
  Related references:
  PMID:31420216 PMID:34707099

• The protein is predominantly localized to the membrane and cytosol, consistent with its roles in signal transduction and apoptosis.

• Key structural features include a transmembrane helix and multiple post-translational modifications (phosphorylation, isopeptide bond, Ubl conjugation), and it requires manganese as a metal cofactor for catalysis.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 380–660) corresponds to a region of PDE3A that includes parts of the catalytic domain. This design may generate antibodies recognizing native PDE3A with potential for functional interference; however, users should validate in their specific assay context.

• For WB applications, a band at ~125 kDa is expected; consider using appropriate controls and verifying with positive cell lysates (e.g., human, mouse, rat).

• For IHC-P and IF/ICC, optimization of antigen retrieval and antibody dilution is recommended, as formalin-fixed paraffin-embedded sections may mask epitopes.

• Although cross-reactivity with human, mouse, and rat is indicated, users should confirm reactivity in their species of interest, especially if using samples with low expression levels.

• The antibody’s performance in ELISA indicates suitability for quantitative detection; pairing with a matched detection antibody is advised for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies diagnostic manufacturers, labs, and research institutes with comprehensive IVD raw material solutions—from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents—streamlining the path from concept to clinic. This PDE3A-targeted antibody supports research into apoptosis, cyclic nucleotide signaling, and related disease mechanisms, and CamelBio can additionally assist in sourcing rare-target raw materials or custom antibody development to meet specialized project needs.