Anti-PDE1A Polyclonal Antibody for WB and ELISA - P54750

Core Product Specifications and Parameters

Biological Background: PDE1A Function and Localization

• PDE1A, also known as HCAM1 or CAM-PDE-1A, is a dual specificity phosphodiesterase that hydrolyzes both cGMP and cAMP, with greater efficiency toward cGMP.
• It is regulated by calcium/calmodulin, linking cyclic nucleotide signaling to intracellular calcium levels.
• The enzyme belongs to the cyclic nucleotide phosphodiesterase family and requires metal ions such as magnesium and zinc for catalytic activity.
• PDE1A is expressed in several tissues including brain, kidney, testes, and heart, indicating roles in diverse physiological processes.
• Alternative splicing of PDE1A produces multiple isoforms, potentially contributing to functional diversity.
• The 61 kDa Cam-PDE (hCam-1) isoform represents the canonical protein referenced by UniProt entry P54750.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 446–545, a region that may be conserved among PDE1A isoforms; consider verifying isoform specificity in your model system.
• This antibody is suitable for WB and ELISA based on supplier data; for WB, a band around 61 kDa is expected, but alternative splicing could generate additional bands.
• Species cross-reactivity is reported for mouse and rat; validate reactivity in your specific strain or tissue of interest.
• Use appropriate negative and positive controls (e.g., PDE1A knockdown lysates or tissues with known expression such as brain) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a comprehensive one-stop sourcing solution for IVD raw materials, from concept to clinic. For PDE1A-related assay development, we supply validated antibody pairs, optimized polyclonal antibodies like the anti-PDE1A Rabbit pAb, and bulk ancillary reagents. Our expertise also covers rare target raw-material sourcing and custom development to accelerate your diagnostic projects.