Anti-PDE12 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q6L8Q7

Core Product Specifications and Parameters

Biological Background: PDE12 Function and Localization

• PDE12 encodes 2',5'-phosphodiesterase 12, also known as mitochondrial deadenylase, which belongs to the 2H phosphodiesterase superfamily. It is involved in the cleavage of 2',5'-phosphodiester bonds in 5'-triphosphorylated oligoadenylates (2-5A), producing AMP and ATP. Related references: PMID:26055709
• The enzyme also cleaves 3',5'-phosphodiester bonds of oligoadenylates, indicating broad substrate specificity. Related references: PMID:21666256, PMID:26055709, PMID:30389976
• PDE12 functions as a negative regulator of the 2-5A system, a key interferon-induced antiviral and antitumor pathway. Suppression of PDE12 elevates cellular 2-5A levels and reduces viral replication. Related references: PMID:26055709
• The protein is localized to the mitochondrial matrix and contains a transit peptide for mitochondrial import.
• PDE12 is ubiquitously expressed across human tissues.
• It requires magnesium ions as a cofactor and is subject to phosphorylation, as indicated by proteomics data.
• Alternative splicing of the PDE12 gene may generate multiple protein isoforms.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a recombinant fusion protein corresponding to amino acids 17-300 of human PDE12 (NP_001309106.1). Consider the conservation of this N-terminal region when planning cross-species experiments.
• The predicted molecular weight of PDE12 is approximately 67 kDa. In Western blot analysis, expect a band near this size and validate specificity with appropriate controls.
• For immunofluorescence and immunocytochemistry, co-stain with mitochondrial markers to confirm localization to the mitochondrial matrix.
• This antibody is compatible with ELISA; optimize coating antigen and detection conditions for your specific assay.
• Reported cross-reactivity with mouse and rat allows use in rodent models; validate reactivity in the tissue of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a streamlined, one-stop platform for IVD raw material procurement, from concept to clinic. For targets such as PDE12, a critical regulator of the antiviral 2-5A pathway, we supply validated monoclonal and polyclonal antibodies, recombinant proteins, and bulk ancillary reagents. We also excel in sourcing rare-target raw materials and offer technical consulting to support every stage of assay development.