Anti-PDE10A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y233

Core Product Specifications and Parameters

Biological Background: PDE10A Function and Localization

• Encoded by the PDE10A gene; multiple isoforms exist via alternative initiation and splicing.
• Functions as a dual-specificity phosphodiesterase, hydrolyzing both cAMP and cGMP with higher affinity for cAMP (PubMed:10373451, 10393245, 17389385). Related references: PMID:10373451 PMID:10393245 PMID:17389385
• Plays a critical role in modulating cAMP and cGMP levels in the striatum, thereby influencing movement and cognition (PubMed:27058447). Related references: PMID:27058447
• Localizes predominantly to the cytoplasm/cytosol.
• Abundantly expressed in the putamen, caudate nucleus, testis, and moderately in thyroid, pituitary, thalamus, and cerebellum.
• Contains cAMP- and cGMP-binding domains and functions as an allosteric enzyme.
• Post-translational modifications include phosphorylation; metal ion binding is required for catalysis.
• Associated with disease variants; listed as a reference proteome member.

Experimental Guidance and Technical Tips

• Based on the immunogen (aa 23-200 of isoform NP_001124162.1), this antibody recognizes a region in the N-terminal portion of PDE10A; confirm sequence alignment across isoforms if specific isoform detection is required.
• For Western blotting, a band near 88 kDa is expected; potential additional bands from alternative splicing or post-translational modifications should be verified.
• In immunocytochemistry/immunofluorescence, permeabilization with Triton X-100 or saponin is recommended to access the cytosolic PDE10A protein.
• For ELISA applications, the polyclonal nature may enhance capture sensitivity, but cross-reactivity to rat PDE10A should be validated in the intended assay matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with streamlined access to IVD raw materials such as validated antibody pairs and optimized monoclonal/polyclonal antibodies. For neuroscience and signaling research involving PDE10A, we offer this high-quality polyclonal antibody alongside bulk ancillary reagents and tailored sourcing of rare targets. Partner with us to accelerate your assay development from concept to clinic.