Anti-PDCD7 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q8N8D1

Core Product Specifications and Parameters

Biological Background: PDCD7 Function and Localization

• Programmed cell death protein 7 (PDCD7), also known as ES18 or hES18, is a nuclear protein that promotes apoptosis when overexpressed.
• It localizes to the nucleus, consistent with its role in apoptosis regulation.
• The protein features a coiled-coil domain and belongs to the reference proteome; its structure has been determined by 3D-structure analysis.
• PDCD7 is classified under keywords: 3D-structure, Apoptosis, Coiled coil, Nucleus, Proteomics identification, Reference proteome, Repeat, indicating its structural and functional attributes.
• The predicted molecular weight of human PDCD7 is approximately 54.7 kDa based on UniProt annotation, consistent with the observed 55 kDa in western blot.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human PDCD7; therefore, the antibody may recognize all isoforms sharing this sequence.
• For western blot (WB), a band around 55 kDa is expected for endogenous PDCD7; use appropriate positive controls (e.g., human, mouse, or rat cells known to express PDCD7) and validate in your sample system.
• For immunohistochemistry (IHC-P), optimize antigen retrieval conditions and consider nuclear staining patterns for PDCD7.
• In ELISA, verify performance with recombinant PDCD7 or peptide to ensure specificity; note that the antibody is raised against a linear peptide epitope.
• As PDCD7 function is linked to apoptosis, consider treating cells with apoptosis inducers to upregulate PDCD7 and confirm antibody specificity under induced conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, providing researchers and diagnostic manufacturers with reliable antibodies and reagents for apoptosis and cell death research. From validated antibody pairs to bulk ancillary reagents, CamelBio streamlines the procurement of high-quality materials for targets like PDCD7. For rare or specialized requirements, our team assists in custom antibody development and rapid sourcing to accelerate your projects from concept to clinic.