Anti-PDCD5 Polyclonal Antibody for WB, IF/ICC, ELISA - O14737

Core Product Specifications and Parameters

Biological Background: PDCD5 Function and Localization

• PDCD5 (Programmed cell death protein 5), also known as TFAR19 (TF-1 cell apoptosis-related protein 19), is a 14 kDa protein encoded by the PDCD5 gene in humans.
• In response to DNA damage, PDCD5 associates with TP53/p53 to prevent MDM2-mediated degradation of TP53, thereby promoting TP53-mediated signaling and apoptosis. Related references: PMID:19616514, PMID:22914926, PMID:28051100
• PDCD5 also protects KAT5/TIP60 from degradation, further enhancing TP53/p53-mediated signaling. Related references: PMID:19308289
• PDCD5 localizes to the nucleus, cytoplasm, and cytosol, allowing it to interact with cytoplasmic and nuclear partners.
• It is widely expressed across tissues, with highest levels in heart, testis, kidney, pituitary gland, adrenal gland, and placenta.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its function and stability.
• Alternative splicing generates multiple PDCD5 isoforms, potentially modulating its activity.
• As its name implies, PDCD5 is involved in programmed cell death (apoptosis), particularly in the context of DNA damage and TP53 activation.

Experimental Guidance and Technical Tips

• For western blotting (WB), note the predicted molecular weight of ~14 kDa and optimize gel percentage and transfer conditions accordingly.
• For immunofluorescence/ICC, consider appropriate permeabilization and fixation methods to detect PDCD5 in its nuclear and cytoplasmic locations.
• As a polyclonal antibody, validate lot-to-lot consistency and performance in your specific sample system.
• The immunogen spans amino acids 1-125 of human PDCD5; cross-reactivity with mouse and rat is indicated, but verify in your experimental model.
• For ELISA, optimize coating and detection conditions using the recombinant protein immunogen as a positive control if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering a comprehensive portfolio of validated antibodies, antigen pairs, and ancillary reagents. This anti-PDCD5 polyclonal antibody supports apoptosis and DNA damage signaling research, with availability in bulk quantities to meet your diagnostic development needs. Leverage our expertise to streamline your supply chain for rare and critical targets like PDCD5 from concept to clinic.