Anti-PDCD4 Rabbit Polyclonal Antibody (KO Validated) for WB, IHC-P, ELISA - Q53EL6

Core Product Specifications and Parameters

Biological Background: PDCD4 Function and Localization

• PDCD4 (Programmed cell death protein 4), also known as H731, D4, Neoplastic transformation inhibitor protein, or Nuclear antigen H731-like, is encoded by the PDCD4 gene in humans. Its canonical isoform has a molecular weight of approximately 52 kDa (UniProt: 51.7 kDa).
• Functioning as a tumor suppressor, PDCD4 inhibits cap-dependent translation by binding to EIF4A and blocking its interaction with EIF4G, thereby suppressing the helicase activity required for translation initiation.
• PDCD4 modulates the JUN kinase pathway and down-regulates MAP4K1 expression, which inhibits MAPK85 activation and JUN-dependent transcription, processes important in driving cellular invasion.
• It plays a role in apoptosis and inhibits tumor promoter-induced neoplastic transformation, consistent with its reduced expression observed in lung cancer and colon carcinoma.
• Localized to both the nucleus and cytoplasm, PDCD4 is up-regulated in proliferative cells and highly expressed in epithelial cells of the mammary gland, suggesting a context-dependent regulatory role.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitination, which may regulate its stability, subcellular localization, and tumor-suppressive functions.
• Alternative splicing generates multiple isoforms, potentially contributing to diverse functional outcomes in different tissues or disease states.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1-260 of human PDCD4 (NP_055271.2). This N-terminal region includes critical domains for protein interactions and functional regulation.
• Consider this antibody for detecting full-length PDCD4 in Western blot (WB), immunohistochemistry (paraffin-embedded sections, IHC-P), and ELISA applications. Validate specificity using knockout (KO) cell lines or tissues, as the product is KO validated.
• Given reported cross-reactivity with human and mouse, confirm reactivity in your specific sample matrix, especially when using mouse tissues for disease models.
• Optimize primary antibody dilution and incubation conditions for each application; for IHC-P, antigen retrieval may be required due to nuclear localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering rapid access to validated antibodies and ancillary reagents for apoptosis and translational research. With a portfolio that includes optimized polyclonal antibodies like this anti-PDCD4 product, as well as bulk reagents and rare-target sourcing, we help diagnostic manufacturers accelerate development from concept to clinic.